Analysis of the Saccharomyces cerevisiae mitochondrial COX3 mRNA 5' untranslated leader: translational activation and mRNA processing.

Molecular and Cellular Biology
G WiesenbergerT D Fox

Abstract

We used transformation of yeast mitochondria and homologous gene replacement to study features of the 613-base COX3 mRNA 5' untranslated leader (5'-UTL) required for translational activation by the protein products of the nuclear genes PET54, PET122, and PET494 in vivo. Elimination of the single AUG triplet in the 5'-UTL had no detectable effect on expression, indicating that activator proteins do not work by allowing ribosomes to bypass that AUG. Deletion of the entire 5'-UTL completely prevented translation, suggesting that the activator proteins do not function by antagonizing any other negative element in the 5'-UTL. Removal of the 15 terminal bases from the 5' end of the 5'-UTL did not block activator-dependent translation. The largest internal deletion that did not interfere with translation removed 125 bases from the upstream portion of the leader. However, two large deletions that blocked translation could be reverted to activator-dependent expression by secondary changes in the remaining 5'-UTL sequences, indicating that the original deletions had not removed the translational activator target but only deformed it. Taken together, the deletion mutations and revertants define a region of 151 bases (between positions -48...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J Conde, G R Fink
Jan 1, 1991·Methods in Enzymology·T D FoxM C Costanzo
Jan 1, 1991·Methods in Enzymology·M P Yaffe
Dec 1, 1990·Trends in Biochemical Sciences·R J JacksonA Kaminski
Jan 1, 1990·Annual Review of Genetics·M C Costanzo, T D Fox
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Jul 1, 1989·European Journal of Biochemistry·L A Grivell
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M C Costanzo, T D Fox
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S AltuviaA B Oppenheim
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Jan 1, 1983·Methods in Enzymology·G Ammerer
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Mar 11, 1980·Nucleic Acids Research·D Gallwitz, R Seidel
Feb 1, 1995·Molecular and Cellular Biology·T M Mittelmeier, C L Dieckmann
May 1, 1994·Molecular Biology Reports·H J Pel, L A Grivell
May 1, 1994·Journal of Virology·G W Witherell, E Wimmer
Jan 1, 1994·International Review of Cytology·C L Dieckmann, R R Staples

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Citations

Aug 27, 2005·Mitochondrion·Jill KleidonRobert Brambl
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·D S LupoldD B Stern
Apr 6, 2018·Biochemistry. Biokhimii︠a︡·K S DerbikovaP A Kamenski
Jun 9, 2006·American Journal of Physiology. Cell Physiology·Flavia FontanesiAntoni Barrientos
Apr 7, 1998·Molecular and Cellular Biology·N S Green-WillmsM C Costanzo
Mar 7, 2020·Biological Chemistry·Leticia Veloso Ribeiro FrancoAlexander Tzagoloff
May 30, 1997·Biochimica Et Biophysica Acta·L I Grossman, M I Lomax

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