Analysis of the structure and expression of the chicken gene encoding a homolog of the human RREB-1 transcription factor

Gene
J H MiyakeW E Stumph

Abstract

Ras proteins are involved in a number of signal transduction pathways including the mitogen-activated kinase cascade. Activated MAPKs translocate to the nucleus and phosphorylate transcription factors such as c-myc, TCF and AP-1. Recently, a Ras-responsive element binding transcription factor, RREB-1, was cloned from a human medullary thyroid carcinoma cell line. RREB-1 is a zinc finger protein that binds to a Ras-responsive element in the promoter of the human calcitonin gene. We report the cloning of the chicken homologue to human RREB-1. Amino-acid alignment demonstrates that chicken and human RREB-1 are 53% identical and 69% similar. Genomic southern analysis indicates that chicken rreb-1 is a single-copy gene in the chicken genome. We demonstrate that chicken and human rreb-1 display the same tissue distribution, being expressed in all tissues examined except the brain. Interestingly, chicken RREB-1 has an extended N-terminus and contains 16 zinc fingers of the TFIIIA subclass, in comparison to human RREB-1 which was reported to contain only four zinc fingers. The size discrepancy between the two predicted gene products is further discussed. An unusual structural feature of RREB-1 is the widely spaced arrangement of the zi...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J R Desjarlais, J M Berg
Feb 1, 1991·Trends in Genetics : TIG·A Gutman, B Wasylyk
Dec 10, 1983·Nucleic Acids Research·T A KostS H Hughes
Feb 16, 1995·Nature·T Pawson
Feb 24, 1995·The Journal of Biological Chemistry·M TowatariT Enver
Mar 1, 1994·Cancer Metastasis Reviews·R Khosravi-Far, C J Der
Feb 7, 1997·Cell·T Hunter
Mar 14, 1997·Science·K IraniP J Goldschmidt-Clermont

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Citations

Mar 20, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M Hollyday
Oct 28, 1999·Clinical Chemistry and Laboratory Medicine : CCLM·S RusswurmK Reinhart
Jan 15, 2014·Differentiation; Research in Biological Diversity·Liang MingHoward D Lipshitz
Jun 28, 2011·The American Journal of Pathology·Matthew D NitzDan Theodorescu
Nov 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sébastien FlajolletPhilippe Moreau

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