Analysis of the structure and substrate binding of Phormidium lapideum alanine dehydrogenase

Nature Structural Biology
P J BakerD W Rice

Abstract

The structure of the hexameric L-alanine dehydrogenase from Phormidium lapideum reveals that the subunit is constructed from two domains, each having the common dinucleotide binding fold. Despite there being no sequence similarity, the fold of alanine dehydrogenase is closely related to that of the family of D-2-hydroxyacid dehydrogenases, with a similar location of the active site, suggesting that these enzymes are related by divergent evolution. L-alanine dehydrogenase and the 2-hydroxyacid dehydrogenases also use equivalent functional groups to promote substrate recognition and catalysis. However, they are arranged differently on the enzyme surface, which has the effect of directing opposite faces of the keto acid to the dinucleotide in each case, forcing a change in absolute configuration of the product.

References

Sep 1, 1975·Archives of Microbiology·P Rowell, W D Stewart
Oct 1, 1979·European Journal of Biochemistry·T Ohashima, K Soda
May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
May 29, 1991·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C R DunnJ J Holbrook
Dec 1, 1991·Journal of Molecular Graphics·C C HuangR Langridge
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jan 15, 1989·European Journal of Biochemistry·A VancuraV Bĕhal
Jan 1, 1985·Methods in Enzymology·B W Matthews, M G Rossmann
May 1, 1974·Journal of Bacteriology·S M McCowen, P V Phibbs
Feb 14, 1971·Journal of Molecular Biology·B Lee, F M Richards
Jan 18, 1969·Nature·C S WrightJ Kraut
Sep 29, 1981·Biochemistry·C E Grimshaw, W W Cleland
Jan 1, 1995·Nature Structural Biology·D J SchullerL J Banaszak
Feb 25, 1994·Journal of Molecular Biology·V S LamzinK S Wilson
Mar 4, 1994·Journal of Molecular Biology·J D GoldbergP Brick
Nov 1, 1993·Journal of Bacteriology·K J SiranosianA D Grossman
Aug 1, 1993·Archives of Biochemistry and Biophysics·M T Smith, D W Emerich
Jan 1, 1993·Protein Engineering·G J Barton
Apr 1, 1997·Journal of Molecular Graphics & Modelling·R M Esnouf
Oct 8, 1998·Acta Crystallographica. Section D, Biological Crystallography·S SedelnikovaP J Baker
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

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Citations

Nov 29, 2011·Applied Microbiology and Biotechnology·Yinxia LiYoshihiro Sawa
Dec 10, 1999·Microbial & Comparative Genomics·W K StudleyM H Saier
May 6, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sarvind Mani Tripathi, Ravishankar Ramachandran
Feb 2, 2002·Applied and Environmental Microbiology·Yusuke TamuraHayao Taguchi
Nov 2, 2004·Journal of Bacteriology·Imke SchröderHarold G Monbouquette
Apr 9, 2008·Bioscience, Biotechnology, and Biochemistry·Yusuke WadaHayao Taguchi
Apr 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Aiguo ZhaoLin Liu
Feb 27, 2007·Biochemical and Biophysical Research Communications·Takeshi ShinodaHayao Taguchi
Jun 8, 2006·Journal of Molecular Biology·Michael P S BoothR Leo Brady
Dec 30, 2014·Protein Engineering, Design & Selection : PEDS·Puja FernandesEmily C Mundorff
Jan 29, 2002·Journal of Molecular Biology·Matthew Bashton, Cyrus Chothia
Mar 23, 2017·Biochemical and Biophysical Research Communications·Nayuta FurukawaHayao Taguchi
Dec 28, 1999·FEBS Letters·J B JacksonS A White
Jan 24, 2006·Journal of Biochemistry·Yoshirou IshikuraHayao Taguchi
Apr 26, 2019·Critical Reviews in Biotechnology·Ushmaben Chandrakantbhai Dave, Ravi-Kumar Kadeppagari
Nov 7, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jinjin WenJiansong Ju
Oct 19, 2016·Biotechnology and Applied Biochemistry·Alexandra LerchnerArne Skerra
Oct 11, 2017·Chembiochem : a European Journal of Chemical Biology·Susana Velasco-LozanoFernando López-Gallego
Aug 16, 2016·Chemistry : a European Journal·Eunice S da SilvaFernando López-Gallego
Mar 16, 2018·The Journal of Biological Chemistry·Prem PrakashPrasenjit Bhaumik

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