PMID: 7525837Nov 1, 1994Paper

Analysis of the structure of empty and peptide-loaded major histocompatibility complex molecules at the cell surface

The Journal of Experimental Medicine
B CatipovićJ P Schneck

Abstract

We compared the conformation of empty and peptide-loaded class I major histocompatibility complex (MHC) molecules at the cell surface. Molecular conformations were analyzed by fluorescence resonance energy transfer (FRET) between fluorescent-labeled Fab fragments bound to the alpha 2 domain of the MHC heavy chain and fluorescent-labeled Fab fragments bound to beta 2-microglobulin. No FRET was found between Fab fragments bound to empty H-2Kb, but FRET was detected when empty H-2Kb molecules were loaded with peptide. The magnitude of FRET depended on the sequence of the peptide used. The results imply that empty H-2Kb molecules are in a relatively extended conformation, and that this conformation becomes more compact when peptide is bound. These changes, which are reflected in peptide-dependent binding of monoclonal antibodies, affect the surfaces of MHC molecules available for contact with T cell receptors and hence may influence T cell-receptor recognition of MHC molecules.

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Citations

Jun 9, 2005·Current Opinion in Microbiology·Alexander GriekspoorJacques Neefjes
Aug 1, 1997·Immunology Today·T Elliott
Oct 12, 2001·Current Opinion in Neurobiology·L M BoulangerC J Shatz
Jun 23, 2004·Nature Reviews. Neuroscience·Lisa M Boulanger, Carla J Shatz
Jul 20, 1996·AIDS Research and Human Retroviruses·C de SantisA Beretta
Dec 1, 1996·Immunological Reviews·J A Frelinger, M McMillan
Oct 1, 2011·Journal of Cellular Physiology·Gordana Blagojević ZagoracPero Lučin
Jun 1, 1997·Protein Science : a Publication of the Protein Society·N FroloffB Honig
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Federico PerosaFranco Dammacco
Aug 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vladimir JankovićJanko Nikolich-Zugich
May 17, 2021·Current Opinion in Immunology·Nouria Jantz-Naeem, Sebastian Springer

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