Analysis of the survival of Venezuelan equine encephalomyelitis virus and possible viral simulants in liquid suspensions

Journal of Applied Microbiology
J E Fitzgibbon, J-L Sagripanti

Abstract

To compare the inactivation rate of Venezuelan equine encephalomyelitis (VEE) virus in liquids to that of Sindbis virus (SV, another alphavirus) and to a bacteriophage (MS2) generally used as a viral simulant in the development of countermeasures in biodefense. Viruses were inoculated into liquids and viral titres were determined at various times postinoculation. The viruses were stable in distilled-deionized (dd) water at 4 degrees C during the 21 days of the study. The inactivation rates of VEE and SV in dd water at 21 and 30 degrees C were very similar (between 0.12 and 0.14 log(10) per day), while MS2 was three-fold slower. In tap water (chlorine content between 4 and 5 ppm) at 21 degrees C, VEE and SV were inactivated at twice the rate measured in dd water. The inactivation rates of VEE and SV were similar to each other and faster than MS2 in all liquids tested. VEE is likely to remain viable for many days after release into water, snow, or even chlorinated tap water. SV can be used to estimate the persistence of VEE in liquids, but using MS2 as a simulant would overestimate of the stability of VEE.

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Citations

Sep 16, 2010·Archives of Virology·Jose-Luis SagripantiLouis E Holland
Aug 16, 2013·Astrobiology·Dale Warren Griffin
Jul 24, 2012·Photochemistry and Photobiology·Jose-Luis SagripantiCarl David Lytle
Aug 23, 2011·Applied and Environmental Microbiology·Jose-Luis SagripantiHans-Jürgen Marschall
Jan 5, 2011·International Journal of Microbiology·Mary Margaret WadeAlan W Zulich
Sep 17, 2020·Journal of Fish Diseases·Mark P PolinskiKyle A Garver
Sep 15, 2020·Resources, Conservation, and Recycling·Sampriti KatakiSanjai K Dwivedi

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