PMID: 8611637Feb 2, 1996Paper

Analysis of the T-cell activation signaling pathway mediated by tyrosine kinases, protein kinase C, and Ras protein, which is modulated by intracellular cyclic AMP

Biochimica Et Biophysica Acta
T OhtsukaT Satoh

Abstract

T-cell receptor (TCR) triggering by an anti-CD3 antibody or phytohemagglutinin (PHA) as well as the treatment with phorbol myristate acetate (PMA), a direct activator of protein kinase C (PKC), induces activation of Ras in T-lymphocytes (Downward, J. et al. (1990)) Nature 364, 719-723). In this paper, we studied the role of Ras in the process of TCR-mediated T-cell activation using a human lymphomic Jurkat cell line. The stimulatory effect of TCR cross-linking on Ras activation was inhibited by herbimycin A, a specific inhibitor of protein tyrosine kinases (PTKs), whereas PMA-induced Ras activation was not affected. On the other hand, calphostin C, a specific inhibitor of PKC, blocked not only PMA-induced, but also TCR-mediated formation of Ras.GTP. Furthermore, down-regulation of PMA-sensitive PKC severely impaired the activation of Ras in response to TCR-stimulation. Tyrosine-phosphorylation and translocation to the particulate fraction of phospholipase C-gamma 1 (PLC-gamma 1) were observed upon T-cell activation. Subcellular localization of PKC was also changed when the cells were stimulated with an anti-CD3 antibody or PMA. While TCR-stimulated translocation of PKC was observed only transiently, PMA-induced translocation of...Continue Reading

References

May 1, 1992·European Journal of Immunology·T Van der Pouw-KraanL Aarden
Mar 1, 1992·Immunology Today·J DownwardD Cantrell
Jan 1, 1991·Methods in Enzymology·Y Uehara, H Fukazawa
Oct 1, 1990·Molecular and Cellular Biology·J SzeberényiG M Cooper
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T J Novak, E V Rothenberg
Aug 23, 1990·Nature·J DownwardD A Cantrell
Mar 15, 1989·Biochemical and Biophysical Research Communications·E KobayashiT Tamaoki
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J B ImbodenJ D Stobo
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L M GravesE G Krebs
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B R SevetsonJ C Lawrence
Nov 12, 1993·Science·S J Cook, F McCormick
Oct 1, 1994·Molecular and Cellular Biology·S HäfnerW Kolch
Jan 1, 1994·Annual Review of Biochemistry·G R Crabtree, N A Clipstone
Jul 1, 1994·Trends in Biochemical Sciences·J AvruchJ M Kyriakis
Aug 1, 1993·Trends in Biochemical Sciences·J Schlessinger
Jan 28, 1994·Cell·A Weiss, D R Littman
Jan 1, 1993·Annual Review of Biochemistry·D R Lowy, B M Willumsen

❮ Previous
Next ❯

Citations

Jan 1, 1997·Immunologic Research·Y Lu, J P Durkin
Jan 5, 2002·Experimental Gerontology·Nadine DouziechTamàs Fulop
Sep 25, 2012·Experimental and Molecular Pathology·Cosmin A TeglaHorea Rus
Jun 8, 2006·Methods in Enzymology·Takaya SatohTohru Kataoka
Jun 9, 2004·Oncogene·José L ZugazaXosé R Bustelo
Jun 11, 1998·The Journal of Biological Chemistry·A BallesterS Alemany
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A NobleD M Kemeny
Jan 10, 2020·Journal of Immunology Research·Michał ZychRadoslaw Zagozdzon
Jul 20, 2021·Chemical Science·Chad PlumetSébastien Papot

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.