PMID: 9185691Jun 11, 1997Paper

Analysis of the T cell response to tumor and viral peptide antigens by an IFNgamma-ELISPOT assay

International Journal of Cancer. Journal International Du Cancer
C ScheibenbogenU Keilholz

Abstract

We have established a sensitive ELISPOT assay measuring interferon gamma (IFN gamma) release on a single-cell basis to detect influenza peptide-specific CD8+ T cells in uncultured peripheral blood mononuclear cells (PBMC). Using this method, we studied the T cell response to HLA-A1 and HLA-A2.1 binding peptide epitopes derived from the MAGE-1 and MAGE-3 proteins, from the melanoma-associated antigens tyrosinase, Melan-A/MART-1 and gp100, and from influenza proteins in stage IV melanoma patients and healthy controls. In 18 of 24 HLA-A2-positive donors (75%), but only in 9 of 25 HLA-A2-positive melanoma patients (36%) T cells reactive with the influenza matrix peptide were demonstrated (p = 0.007). T cells responding to one or several of the melanoma-associated peptides were detected in 5 of 25 HLA-A2-positive patients with metastatic melanoma. Four of these 5 patients had been treated with interleukin-2- and IFN alpha-containing therapy. Two of the 24 healthy donors had T cells reactive with the MART-1 27-35 peptide. No reactivity with the HLA-A1-binding peptides from MAGE-1 or MAGE-3 was detected in any of the HLA-A1-positive healthy controls or melanoma patients. These results show that the IFN gamma-ELISPOT assay is suitable ...Continue Reading

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Citations

Sep 24, 2004·The Journal of Experimental Medicine·Mary Jo TurkAlan N Houghton
Mar 1, 2005·Journal of Neuro-oncology·A RousseauJ Choppin
Jul 12, 2008·Cancer Immunology, Immunotherapy : CII·E H J G AarntzenI J M de Vries
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Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Dieter KabelitzMargot Zöller
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