Analysis of the Tcf-3 promoter during early development of Xenopus

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Nicole SpiekerOlivier Destrée

Abstract

XTcf-3 functions as a transcriptional regulator in the canonical Wnt signaling cascade and can repress or activate downstream target genes. Expression of XTcf-3 is differentially regulated in time and place during development (Molenaar et al. [1998] Mech Dev. 75:151-154), but little is known about the mechanisms that control transcriptional activation and repression. A 15-kb genomic fragment of Tcf-3 sequences from Xenopus tropicalis was cloned, including the 5' untranslated region; exons 1, 2, and 3; and intron sequences. We used 5' deletion constructs for transgenesis and episomal luciferase assays in Xenopus to examine temporal and spatial regulation of the promoter during early development. A -3054/+34-bp Tcf-3 upstream region was identified that drives a green fluorescent protein (GFP) reporter transgene in a pattern similar to endogenous expression of XtTcf-3 from gastrula to tail bud stages. At stage 12, expression of the reporter is restricted to the middle and posterior neurectoderm. At stage 22, expression is strongest in the neural plate, the eye anlagen and branchial arches. At stage 35/36, expression is found in the head mesenchyme, the branchial arches, the heart, the mesencephalon, eyes, otic vesicles, notochord,...Continue Reading

References

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Mar 25, 1999·Genes & Development·J GalceranR Grosschedl
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Feb 16, 2002·Developmental Biology·Irina StanchevaRichard R Meehan
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Mar 7, 2003·Genome Biology·Joanna KaczynskiRaul Urrutia
Mar 19, 2003·Development·Richard I DorskyAjay Chitnis

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Citations

Feb 8, 2008·Genesis : the Journal of Genetics and Development·Sabrina F KoenigDietmar Gradl
Sep 22, 2011·Molecular and Cellular Biochemistry·Nina SolbergStefan Krauss
May 28, 2019·Cell Communication and Signaling : CCS·Wenyu HuYinan Zhao

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