PMID: 9420250Jan 7, 1998Paper

Analysis of the temporal relationship between human immunodeficiency virus type 1 quasispecies in sequential blood samples and various organs obtained at autopsy

Journal of Virology
A B van't WoutH Schuitemaker

Abstract

We studied the temporal relationship between human immunodeficiency type 1 (HIV-1) quasispecies in tissues and in peripheral blood mononuclear cells (PBMC) of infected individuals. Sequential PBMC and tissue samples from various organs obtained at autopsy from three patients who died of AIDS-related complications were available for analysis. Biological HIV-1 clones were isolated from PBMC samples, and cellular tropism and syncytium-inducing (SI) capacity were determined. Genomic DNA was isolated from 1 cm3 of organ tissue, and proviral DNA was amplified by means of PCR and cloned with the PGEM-T vector system. A 185-bp region encompassing the third variable domain of the virus envelope, known to influence HIV-1 biological properties, was sequenced. HIV-1 could be amplified from all PBMC and organ samples, except from liver tissue for two patients. Both SI and non-syncytium-inducing (NSI) genotypes could be detected in the different tissues. Tissue-specific quasispecies were observed in brain, lung, and testis. Lymphoid tissues, such as bone marrow, lymph node, and spleen, harbored several different variants similar to those detected in blood in the last PBMC samples. In general, only tissues in which macrophages are likely to b...Continue Reading

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Citations

May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·N Dejucq, B Jégou
Aug 22, 2008·Journal of Virology·Marta Sanz-RamosNoemí Sevilla
Jan 30, 2010·Archives of Virology·Jessica J Hayward, Allen G Rodrigo
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Mar 14, 2007·Evolution; International Journal of Organic Evolution·Antonio V BorderíaRafael Sanjuán

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