Analysis of the tobacco chloroplast DNA replication origin (oriB) downstream of the 23 S rRNA gene

Journal of Molecular Biology
M KunnimalaiyaanB L Nielsen

Abstract

We have mapped the origin of DNA replication (oriB) downstream of the 23 S rRNA gene in each copy of the inverted repeat (IR) of tobacco chloroplast DNA between positions 130,502 and 131,924 (IR(A)) by a combination of approaches. In vivo chloroplast DNA replication intermediates were examined by two-dimensional agarose gel electrophoresis. Extended arc patterns suggestive of replication intermediates containing extended single-stranded regions were observed with the 4.29 kb SspI fragment and an overlapping EcoRI fragment from one end of the inverted repeat, while only simple Y patterns were observed with a 3.92 kb BamHI-KpnI fragment internal to the SspI fragment. Other restriction fragments of tobacco chloroplast DNA besides those at the oriA region also generated only simple Y patterns in two-dimensional agarose gels. Several chloroplast DNA clones from this region were tested for their ability to support in vitro DNA replication using a partially purified chloroplast protein fraction. Templates with a deletion of 154 bp from the SspI to the BamHI sites near the end of the inverted repeat resulted in a considerable loss of in vitro DNA replication activity. These results support the presence of a replication origin at the en...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1975·Biochimica Et Biophysica Acta·R Kolodner, K K Tewari
Apr 22, 1992·Proceedings. Biological Sciences·A G MayhookH T Jacobs
Dec 1, 1992·Microbiological Reviews·D LaneM Chandler
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H CeruttiA T Jagendorf
Mar 1, 1992·Molecular & General Genetics : MGG·W L Chiu, B B Sears
Mar 1, 1992·Molecular & General Genetics : MGG·Y TakedaT Nagata
Jan 1, 1991·Annual Review of Cell Biology·D A Clayton
Feb 11, 1990·Nucleic Acids Research·M H Linskens, J A Huberman
Mar 1, 1988·Molecular and Cellular Biology·R MeekerK K Tewari
Jun 24, 1988·Nucleic Acids Research·N Carrillo, L Bogorad
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D D Chang, D A Clayton
Jan 1, 1985·Annual Review of Genetics·J D Palmer
Jul 21, 1995·The Journal of Biological Chemistry·S Schaper, W Messer
Mar 15, 1994·European Journal of Biochemistry·M K ReddyK K Tewari
Nov 1, 1993·Plant Molecular Biology·L A HedrickG C Cannon
Nov 1, 1993·Plasmid·B L NielsenK K Tewari
May 1, 1993·Plant Physiology·H. Cerutti, A. T. Jagendorf
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·X W DengW Gruissem
Jan 1, 1985·Plant Molecular Biology·S HeinhorstA Weissbach

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Citations

Dec 18, 2004·Plant Molecular Biology·Shashi KumarHenry Daniell
Jul 19, 2002·Molecular Microbiology·D H WilliamsonR J M Wilson
Feb 12, 2009·Nucleic Acids Research·Siu Kai Leung, Joseph T Y Wong
Aug 10, 2006·Plant Molecular Biology·Lars B Scharff, Hans-Ulrich Koop
May 2, 2007·The Plant Journal : for Cell and Molecular Biology·Lars B Scharff, Hans-Ulrich Koop
Oct 18, 2002·The Plant Journal : for Cell and Molecular Biology·Stefan K MühlbauerHans-Ulrich Koop
Sep 15, 1997·Nucleic Acids Research·M Kunnimalaiyaan, B L Nielsen
Dec 31, 2003·Journal of Molecular Biology·Delene J Oldenburg, Arnold J Bendich

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