Analysis of the two subgenomic RNA promoters for turnip crinkle virus in vivo and in vitro

Virology
J Wang, A E Simon

Abstract

Infection of plants or protoplasts with turnip crinkle virus (TCV), a monopartite RNA virus, results in the synthesis of the genomic RNA and two subgenomic (sg) RNAs. The transcription start site for the 1.45-kb sgRNA was previously mapped to position 2606 (J. C. Carrington, T. J. Morris, P. G. Stockley, and S. C. Harrison, (1987). J. Mol. Biol. 194, 265-276) corresponding to position 2607 in the TCVms isolate and the start site for the 1.7-kb sgRNA has now been mapped to position 2333 in TCVms. A 96-base sequence (90 bases upstream and 6 bases downstream) encompassing the transcription start site for the 1.45-kb sgRNA was sufficient for full promoter activity. Similarly, a 94-base sequence (90 bases upstream and 4 bases downstream) encompassing the start site was required for full activity of the 1.7-kb sgRNA promoter. The 1.45-kb sgRNA promoter, but not the 1.7-kb sgRNA promoter, was able to direct synthesis of a nontemplate RNA in vitro using partially purified TCV RNA-dependent RNA polymerase. Computer generated secondary structures for the two sgRNA promoters revealed an extensive hairpin just upstream from the transcription start site. Comparisons of corresponding sequences from related viruses indicates higher sequence c...Continue Reading

References

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Citations

Mar 26, 2010·Journal of Virology·Luis Martínez-GilIsmael Mingarro
Jun 14, 2000·Journal of Virology·G Koev, W A Miller
Jan 1, 2001·Molecular Plant Pathology·M AkgozK K Wobbe
Oct 29, 2010·Virus Research·Aurora CastañoCarmen Hernández
Aug 28, 2003·The Plant Journal : for Cell and Molecular Biology·Fengli Zhang, Anne E Simon
Jul 13, 2000·Virology·W A Miller, G Koev
Jan 28, 2003·Journal of Molecular Biology·Guohua Zhang, Anne E Simon
Feb 27, 2003·The Journal of General Virology·Silvia García-CastilloVicente Pallás

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