Analysis of the vaginal microbiome of giant pandas using metagenomics sequencing.

MicrobiologyOpen
Lan ZhangXin Yang

Abstract

In this study, a total of 14 vaginal samples (GPV1-14) from giant pandas were analyzed. These vaginal samples were divided into two groups as per the region and age of giant pandas. All the vaginal samples were analyzed using metagenomic sequencing. As per the outcomes of metagenomic analysis, Proteobacteria (39.04%), Firmicutes (5.27%), Actinobacteria (2.94%), and Basidiomycota (2.77%) were found to be the dominant phyla in the microbiome of the vaginal samples. At the genus level, Pseudomonas (21.90%) was found to be the most dominant genus, followed by Streptococcus (3.47%), Psychrobacter (1.89%), and Proteus (1.38%). Metastats analysis of the microbial species in the vaginal samples of giant pandas from Wolong Nature Reserve, Dujiangyan and Ningbo Youngor Zoo, and Ya'an Bifengxia Nature Reserve was found to be significantly different (p < 0.05). Age groups, that is, AGE1 (5-10 years old) and AGE2 (11-16 years old), also demonstrated significantly different inter-group microbial species (p < 0.05). For the first time, Chlamydia and Neisseria gonorrhoeae were detected in giant pandas' reproductive tract. GPV3 vaginal sample (2.63%) showed highest Chlamydia content followed by GPV14 (0.91%), and GPV7 (0.62%). GPV5 vaginal samp...Continue Reading

References

Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·W U Knudtson, C A Kirkbride
Aug 9, 1996·The Journal of Biological Chemistry·C C Smart, A J Fleming
Aug 7, 1998·Biochimica Et Biophysica Acta·H W van Veen, W N Konings
Nov 13, 2004·American Journal of Primatology·Ismar A MoraesAna Maria R Ferreira
Aug 20, 2005·PLoS Computational Biology·Kevin Chen, Lior Pachter
Dec 31, 2005·Nucleic Acids Research·Minoru KanehisaMika Hirakawa
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Apr 11, 2009·PLoS Computational Biology·James Robert WhiteMihai Pop
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Christian M PaumiSusan Michaelis
Feb 15, 2011·Veterinary Research·Katelijn Schautteet, Daisy Vanrompay
Jun 22, 2011·Genome Research·Daniel H HusonStephan C Schuster
Jun 28, 2011·Genome Biology·Nicola SegataCurtis Huttenhower
Sep 13, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Yingguo LiZhengguo Li
Oct 4, 2011·BMC Bioinformatics·Brian D OndovAdam M Phillippy
Sep 14, 2012·The Biochemical Journal·Terry L SasserRutilio A Fratti
Oct 13, 2012·Bioinformatics·Limin FuWeizhong Li
Dec 4, 2012·The Journal of Allergy and Clinical Immunology·Magali Noval RivasTalal A Chatila
Dec 6, 2012·Nature Communications·Fredrik H KarlssonJens Nielsen
Feb 13, 2013·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Meghan B AzadUNKNOWN CHILD Study Investigators
Feb 23, 2013·Veterinary Microbiology·Claudia CafarchiaDomenico Otranto
May 31, 2013·Nature·Fredrik H KarlssonFredrik Bäckhed
Aug 30, 2013·Nature·Emmanuelle Le ChatelierOluf Pedersen
Aug 30, 2013·Nature·Aurélie CotillardStanislav Dusko Ehrlich
Oct 30, 2013·American Journal of Physical Anthropology·Rebecca M StumpfSteven R Leigh
Nov 12, 2013·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Dec 4, 2013·Nucleic Acids Research·Sean PowellPeer Bork
Jul 7, 2014·Nature Biotechnology·Junhua LiUNKNOWN MetaHIT Consortium
Oct 30, 2014·Microbial Ecology·Nicholas A BeCrystal J Jaing
Nov 18, 2014·Nature Methods·Benjamin BuchfinkDaniel H Huson
Nov 30, 2014·Molecular Systems Biology·Georg ZellerPeer Bork
May 15, 2015·Cell Host & Microbe·Fredrik BäckhedWang Jun
May 23, 2015·Science·Shinichi SunagawaPeer Bork
May 23, 2015·Science·Jennifer R BrumMatthew B Sullivan
Jul 2, 2016·Infectious Diseases in Obstetrics and Gynecology·Philip A ChanErica J Hardy
Jul 29, 2016·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Xin YangGuo Li

❮ Previous
Next ❯

Methods Mentioned

BETA
amplicon sequencing
electrophoresis
Illumina
PCR
PCA
phosphotransferase

Software Mentioned

CD
SOAPdenovo
MEGAN
MetaGeneMark
NMDS
Metastats
R ade4
LEfSe
HIT
ANOSIM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.