PMID: 6168870Aug 1, 1981Paper

Analysis of thick brain sections by obverse-reverse computer microscopy: application of a new, high clarity Golgi-Nissl stain

Journal of Neuroscience Methods
E M Glaser, H Van der Loos

Abstract

Exceptionally clear Golgi-Nissl sections of 300 micron thickness have been morphometrically studied by light microscopy using oil immersion objectives. The clarity results from a new variation of a staining procedure that combines Golgi and Nissl images in one section. A viewing technique has been developed that permits a histologic preparation to be examined from its obverse (or normally viewed) side and its reverse (or under) side. The technique was designed for use with a computer microscope but can be employed with any light microscope whose stage position can be measured within 100 micron. Sections thicker than 300 micron can be studied dependent on the working distance of the objective lens, provided that the clarity of the material permits it.

References

Jan 1, 1965·IEEE Transactions on Bio-medical Engineering·E M GLASER, H VANDERLOOS

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Citations

Sep 25, 2004·Hippocampus·J A MarkhamJ M Juraska
Apr 5, 2011·Brain Structure & Function·Saturnino SpigaMarco Diana
May 1, 1983·Journal of Neuroscience Methods·E M GlaserH Van der Loos
Dec 1, 1986·Journal of Neuroscience Methods·S CatsicasG M Innocenti
Oct 1, 1986·Journal of Neuroscience Methods·H B UylingsJ van Pelt
Mar 1, 1990·Journal of Neuroscience Methods·E KolodziejczykM R Fernandez-Graf
Aug 8, 1983·Neuroscience Letters·P J Berbel, A Fairén
Dec 9, 2003·Journal of Neuroscience Methods·Gorazd RosoklijaAndrew J Dwork
Jun 24, 2004·Neurobiology of Aging·Sarah J WorksCara L Wellman
Jan 5, 2002·Journal of Neuroscience Methods·D A Tata, B J Anderson
Feb 13, 2003·Journal of Neuroscience Methods·Bassam N Hamam, Timothy E Kennedy
Apr 8, 1998·Journal of Neuroscience Methods·R Gibb, B Kolb
May 29, 1998·Brain Research. Developmental Brain Research·M K JarvinenT L Powley
May 16, 2002·Neurobiology of Aging·Julie A Markham, Janice M Juraska
Jan 14, 2003·Neuroscience Letters·Laura M Seib, Cara L Wellman
May 25, 2004·Nature Neuroscience·Stuart K Amateau, Margaret M McCarthy
Aug 13, 2011·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Marco BortolatoJean C Shih
Feb 16, 2012·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Marguerite C CampAndrew Holmes
Nov 20, 2008·Proceedings of the National Academy of Sciences of the United States of America·Douglas A MeyerJames A Bibb
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chengwei HeJing X Kang
Feb 12, 2009·The Journal of Biological Chemistry·Corinna SawallischHans-Jürgen Kreienkamp

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