Analysis of three human interleukin 5 structures suggests a possible receptor binding mechanism

FEBS Letters
J L VerscheldeJ Tavernier

Abstract

We compared three crystal structures of human interleukin 5 (hIL5) expressed in either E. coli (hIL5E.coli), Sf9 cells (hIL5sf9) or Drosophila cells (hIL5Drosophila). The dimeric hIL5 structures show subtle but significant conformational differences which are probably a consequence of the different crystallization conditions trapping this protein into one of two states. We refer to these two distinct conformations as the 'open' and 'tight' state, according to the packing around the cleft between the two subunits. We hypothesize that these two stable conformational states reflect the structure of the free or receptor bound hIL5.

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Citations

Apr 12, 2001·European Journal of Immunology·L ZabeauJ Tavernier
May 5, 1999·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·T LalaniA R Ahmed

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