Analysis of topoisomerase function in bacterial replication fork movement: use of DNA microarrays

Proceedings of the National Academy of Sciences of the United States of America
Arkady B KhodurskyNicholas R Cozzarelli

Abstract

We used DNA microarrays of the Escherichia coli genome to trace the progression of chromosomal replication forks in synchronized cells. We found that both DNA gyrase and topoisomerase IV (topo IV) promote replication fork progression. When both enzymes were inhibited, the replication fork stopped rapidly. The elongation rate with topo IV alone was 1/3 of normal. Genetic data confirmed and extended these results. Inactivation of gyrase alone caused a slow stop of replication. Topo IV activity was sufficient to prevent accumulation of (+) supercoils in plasmid DNA in vivo, suggesting that topo IV can promote replication by removing (+) supercoils in front of the chromosomal fork.

References

Sep 1, 1990·Journal of Bacteriology·M B Schmid
Jul 20, 1989·Journal of Molecular Biology·R A Kim, J C Wang
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L F Liu, J C Wang
Jan 1, 1970·Molecular & General Genetics : MGG·P L Carl
Jan 1, 1982·Molecular & General Genetics : MGG·V N KsenzenkoA A Bayev
Jan 1, 1982·Laboratory Animals·C Moolenbeek
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·A B KhodurskyN R Cozzarelli
Jan 1, 1996·Annual Review of Biochemistry·J C Wang
Mar 28, 1997·Journal of Molecular Biology·V V RybenkovN R Cozzarelli
Sep 5, 1997·Science·F R BlattnerY Shao
Sep 30, 1998·Cell·B J PeterN R Cozzarelli
Oct 9, 1998·The Journal of Biological Chemistry·A B Khodursky, N R Cozzarelli
Oct 22, 1998·Molecular Microbiology·P Staczek, N P Higgins
Feb 23, 1999·Biochemistry·S C KampranisA Maxwell
Sep 2, 1999·Nature Genetics·J R PollackP O Brown
Sep 25, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L PostowN R Cozzarelli

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Citations

Aug 2, 2012·Quarterly Reviews of Biophysics·Jonathan M FoggLynn Zechiedrich
Feb 13, 2013·Nature Reviews. Genetics·Xindan WangDavid Z Rudner
Nov 24, 2011·Nature Reviews. Molecular Cell Biology·Seychelle M VosJames M Berger
Apr 12, 2001·Molecular Microbiology·K Hjort, R Bernander
Nov 2, 2002·Molecular Microbiology·L OlavarrietaJ B Schvartzman
Jan 10, 2003·Molecular Microbiology·Tanya D Sokolsky, Tania A Baker
Aug 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bénédicte MichelVladimir Bidnenko
Jan 11, 2000·Nucleic Acids Research·K WatanabeH Kasai
May 10, 2002·Nucleic Acids Research·U H ManjunathaV Nagaraja
Sep 4, 2008·Genes & Development·Xindan WangDavid J Sherratt
Mar 29, 2001·Genes & Development·R W DeiblerE L Zechiedrich
Nov 23, 2006·Antimicrobial Agents and Chemotherapy·Daniel E RozenKeith P Klugman
Aug 8, 2012·Antimicrobial Agents and Chemotherapy·Sandra Aedo, Yuk-Ching Tse-Dinh
Feb 27, 2003·Antimicrobial Agents and Chemotherapy·Christine D Hardy, Nicholas R Cozzarelli
Aug 30, 2005·Antimicrobial Agents and Chemotherapy·Mika Fujimoto-NakamuraJun-Ichi Yamagishi
Dec 27, 2005·Antimicrobial Agents and Chemotherapy·Yoshihiro OyamadaMasaaki Wachi
Jul 27, 2002·Journal of Bacteriology·Wen DengRobert D Perry
Mar 6, 2003·Journal of Bacteriology·Joseph E PetersNancy L Craig
Feb 6, 2004·Journal of Bacteriology·Gianfranco GromponeBénédicte Michel
Jul 1, 2005·BMC Cell Biology·Judita MascarenhasPeter L Graumann
Jun 14, 2006·BMC Microbiology·Devang ShahKim Lewis
Nov 13, 2004·Genome Biology·Kyeong Soo JeongArkady B Khodursky
Nov 13, 2004·Genome Biology·Brian J PeterNicholas R Cozzarelli
May 5, 2005·PLoS Biology·Ryan T CirzFloyd E Romesberg
Oct 3, 2006·PLoS Genetics·Kyeong Soo JeongArkady B Khodursky
Jan 1, 2014·PloS One·Andrea L EdwardsValentin V Rybenkov
Dec 20, 2003·Pharmacogenomics·Dezhong YinYinduo Ji
Mar 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Adam M BreierNicholas R Cozzarelli

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