Analysis of trace levels of sulfonamide and tetracycline antimicrobials in groundwater and surface water using solid-phase extraction and liquid chromatography/mass spectrometry

Analytical Chemistry
M E LindseyE Michael Thurman

Abstract

A method has been developed for the trace analysis of two classes of antimicrobials consisting of six sulfonamides (SAs) and five tetracyclines (TCs), which commonly are used for veterinary purposes and agricultural feed additives and are suspected to leach into ground and surface water. The method used solid-phase extraction and liquid chromatography/mass spectrometry (LC/MS) with positive ion electrospray. The unique combination of a metal chelation agent (Na2EDTA) with a macroporous copolymer resulted in quantitative recoveries by solid-phase extraction (mean recovery, 98 +/- 12%) at submicrogramper-liter concentrations. An ammonium formate/formic acid buffer with a methanol/water gradient was used to separate the antimicrobials and to optimize the signal intensity. Mass spectral fragmentation and ionization characteristics were determined for each class of compounds for unequivocal identification. For all SAs, a characteristic m/z 156 ion representing the sulfanilyl fragment was identified. TCs exhibited neutral losses of 17 amu resulting from the loss of ammonia and 35 amu from the subsequent loss of water. Unusual matrix effects were seen only for TCs in this first survey of groundwater and surface water samples from site...Continue Reading

References

May 1, 1987·Reviews of Infectious Diseases·H L DuPont, J H Steele
Aug 6, 1998·Journal of Chromatography. a·R W Fedeniuk, P J Shand
Aug 6, 1998·Journal of Chromatography. a·F J Schenck, P S Callery
Aug 11, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·M C CarsonS W Hadley
Aug 24, 2000·Journal of Agricultural and Food Chemistry·J KurittuM Karp

❮ Previous
Next ❯

Citations

Jul 19, 2006·Analytical and Bioanalytical Chemistry·Sung-Chul Kim, Kenneth Carlson
Apr 22, 2008·Analytical and Bioanalytical Chemistry·Delphine DebayleMarie Florence Grenier-Loustalot
Jul 28, 2009·Analytical and Bioanalytical Chemistry·María Cruz Moreno-BondiErika Rodriguez
Sep 19, 2008·Archives of Environmental Contamination and Toxicology·Sandra L Kuchta, Allan J Cessna
Jun 11, 2010·Archives of Environmental Contamination and Toxicology·Marco Antonio F LocatelliWilson F Jardim
Jun 24, 2008·Environmental Monitoring and Assessment·Magdalena Velicu, Rominder Suri
Mar 7, 2012·Environmental Science and Pollution Research International·Alejandra IglesiasAlberto Cepeda
Aug 21, 2012·Environmental Science and Pollution Research International·Yuan ZhangYucheng Chen
May 17, 2013·Environmental Science and Pollution Research International·Haiyan ChenChun Zhao
Mar 19, 2013·Chemosphere·Hyunook KimSung-il Cho
Apr 10, 2004·Journal of Chromatography. a·Ning ZhengMei-Juan Wen
Aug 16, 2005·Journal of the American Society for Mass Spectrometry·Thomas PfeiferRegine Fuchs
Nov 4, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Roongnapa SuedeeUrairut Kongmark
Aug 26, 2011·Journal of Agricultural and Food Chemistry·Xiaolin LiMichael Katterhenry
Oct 7, 2003·Environmental Health Perspectives·Gerd HamscherJörg Hartung
May 30, 2009·Environmental Health Perspectives·Pedro A SeguraSébastien Sauvé

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.