Analysis of trace metal distribution in plants with lab-based microscopic X-ray fluorescence imaging

Plant Methods
Ana MijovilovichHendrik Küpper

Abstract

Many metals are essential for plants and humans. Knowledge of metal distribution in plant tissues in vivo contributes to the understanding of physiological mechanisms of metal uptake, accumulation and sequestration. For those studies, X-rays are a non-destructive tool, especially suited to study metals in plants. We present microfluorescence imaging of trace elements in living plants using a customized benchtop X-ray fluorescence machine. The system was optimized by additional detector shielding to minimize stray counts, and by a custom-made measuring chamber to ensure sample integrity. Protocols of data recording and analysis were optimised to minimise artefacts. We show that Zn distribution maps of whole leaves in high resolution are easily attainable in the hyperaccumulator Noccaea caerulescens. The sensitivity of the method was further shown by analysis of micro- (Cu, Ni, Fe, Zn) and macronutrients (Ca, K) in non-hyperaccumulating crop plants (soybean roots and pepper leaves), which could be obtained in high resolution for scan areas of several millimetres. This allows to study trace metal distribution in shoots and roots with a wide overview of the object, and thus avoids making conclusions based on singular features of ti...Continue Reading

References

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Nov 30, 2018·Frontiers in Plant Science·Eduardo S RodriguesHudson W P Carvalho

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Citations

Aug 26, 2020·Journal of Experimental Botany·Rosario Castro-RodríguezManuel González-Guerrero
Feb 6, 2021·Journal of Experimental Botany·Filis MorinaHendrik Küpper
Aug 19, 2021·The Plant Journal : for Cell and Molecular Biology·Sophie M BrouwerErik Andreasson

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Methods Mentioned

BETA
X-ray
fluorescence imaging
scanning
electron

Software Mentioned

Fiji
Originlab
Xmethod
Origin Professional
M4
ImageJ
macOS
TORNADO

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