Analysis of transcription of the Staphylococcus aureus aerobic class Ib and anaerobic class III ribonucleotide reductase genes in response to oxygen

Journal of Bacteriology
Mahmud MasalhaGerald Cohen


Staphylococcus aureus is a gram-positive facultative aerobe that can grow in the absence of oxygen by fermentation or by using an alternative electron acceptor. To investigate the mechanism by which S. aureus is able to adapt to changes in oxygen concentration, we analyzed the transcriptional regulation of genes that encode the aerobic class Ib and anaerobic class III ribonucleotide reductase (RNR) systems that are responsible for the synthesis of deoxyribonucleotides needed for DNA synthesis. The S. aureus class Ib RNR nrdIEF and class III RNR nrdDG genes and their regulatory regions were cloned and sequenced. Inactivation of the nrdDG genes showed that the class III RNR is essential for anaerobic growth. Inhibition of aerobic growth by hydroxyurea showed that the class Ib RNR is an oxygen-dependent enzyme. Northern blot analysis and primer extension analysis demonstrated that transcription of class III nrdDG genes is regulated by oxygen concentration and was at least 10-fold higher under anaerobic than under aerobic conditions. In contrast, no significant effect of oxygen concentration was found on the transcription of class Ib nrdIEF genes. Disruption or deletion of S. aureus nrdDG genes caused up to a fivefold increase in n...Continue Reading


Jan 1, 1991·Methods in Enzymology·R P Novick
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J CarlsonJ Messing
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·A JordanPeter Reichard
Jan 17, 1995·Biochemical and Biophysical Research Communications·D S King, Peter Reichard
Jan 1, 1996·Methods in Enzymology·D G HigginsT J Gibson
Oct 25, 1996·The Journal of Biological Chemistry·R EliassonPeter Reichard
Dec 4, 1996·Biochemical and Biophysical Research Communications·X GarrigaPeter Reichard
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·V LazarevicD Karamata
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Oct 6, 1998·Annual Review of Biochemistry·A Jordan, Peter Reichard
Dec 10, 1998·Microbiology·V VagnerS D Ehrlich
Apr 14, 1999·Molecular Microbiology·D O GostickJ R Guest
May 14, 1999·Current Opinion in Microbiology·G Sawers
Nov 5, 1999·Trends in Microbiology·M O Clements, Simon J Foster
Nov 5, 1999·Annual Review of Microbiology·C E BauerT H Bird
Nov 7, 1999·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·E MulliezM Fontecave
Apr 5, 2000·The Journal of Biological Chemistry·J AnderssonB M Sjoberg
May 2, 2000·Journal of Clinical Microbiology·J M Yarwood, P M Schlievert
Feb 24, 2001·Molecular and Cellular Biochemistry·R D SinghN K Ganguly
Feb 27, 2001·Journal of Bacteriology·P GiachinoM Bischoff
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J J FerrettiR McLaughlin


Jun 14, 2005·Trends in Genetics : TIG·Dmitry A Rodionov, Mikhail S Gelfand
Jun 15, 2006·Antioxidants & Redox Signaling·Stéphanie Gon, Jon Beckwith
Jul 15, 2004·Nucleic Acids Research·Liangsu WangCarlos Zamudio
Aug 3, 2010·Journal of Bacteriology·Inbal RabinovitchYair Aharonowitz
Jun 15, 2007·Journal of Bacteriology·Julia MakhlinYair Aharonowitz
Feb 3, 2006·Journal of Bacteriology·Wook ChangWilliam E Bentley
Feb 20, 2009·Journal of Bioinformatics and Computational Biology·Guojun LiYing Xu
Jun 8, 2006·Annual Review of Biochemistry·Pär Nordlund, Peter Reichard
Feb 9, 2013·BMC Evolutionary Biology·Bhakti DwivediMya Breitbart
Nov 28, 2013·International Journal of Systematic and Evolutionary Microbiology·Itay AmirAbraham Weinberger
Mar 3, 2018·Toxins·Miloslava DuracovaJiri Dresler
Jan 27, 2016·Journal of Bacteriology·Ian H WindhamKenneth W Bayles
Jan 31, 2019·Future Medicinal Chemistry·Youjun XuJianfeng Pei
Dec 14, 2019·Microbiology Resource Announcements·Ashika Singh-MoodleyArshad Ismail
Dec 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vlad Tiberiu AlexaDaniela Jumanca

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.