Analysis of triglyceride isomers by silver-ion high-performance liquid chromatography. Effect of column temperature on retention times

Journal of Chromatography. a
R O Adlof, G List

Abstract

Silver ion chromatography (Ag-HPLC), utilizing columns packed with silver ions bonded to a silica or similar substrate, has proven to be a powerful technique for the analytical separation of cis and trans geometric and positional fatty acid methyl ester (FAME) and triacylglycerol (TAG) isomers. In this manuscript, we utilize an HPLC column chiller/heater to study the effects of Ag-HPLC column temperatures on elution rates of FAME and TAG isomers. Two Varian ChromSpher lipids columns connected in series and isocratic solvent systems of 1.0% or 1.5% acetonitrile (ACN) in hexane were used to analyze FAMEs (zero to six double bonds; cis/trans isomers), TAGs (homogeneous (triacetyl-, tristearoyl-, trioleoyl-, trilinoeyl- and trilinolenoyl-glycerols) and positional (1,3-distearoyl,2-monolinolenoyl- and 1,2-distearoyl, 3-monolinolenoylglycerol, etc.)) mixtures at four different temperatures (10 degrees C, 20 degrees C, 30 degrees C or 40 degrees C). Unexpectedly, the unsaturated FAME and TAG samples were found to elute more slowly at higher temperatures, a result just the opposite to the usual temperature effect (where samples elute more rapidly at higher temperatures) noted in gas and most liquid (reversed-phase or silica gel substra...Continue Reading

Citations

Aug 15, 2019·Journal of Mass Spectrometry : JMS·Ethan YangPierre Chaurand
Nov 13, 2009·Rapid Communications in Mass Spectrometry : RCM·Corinne Loutelier-BourhisCatherine M Lange
Dec 14, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kentaro YogoKiyokatsu Jinno
Sep 15, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Svetlana M Momchilova, Boryana M Nikolova-Damyanova
Sep 18, 2014·Journal of Mass Spectrometry : JMS·Luca BonoEmanuele Magi
Jul 11, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ikuma KurodaShun Wada
Oct 16, 2012·Analytica Chimica Acta·Zarah WalshMirek Macka
May 29, 2014·Journal of the American Society for Mass Spectrometry·J Stuart GrossertJeremy E Melanson
Jan 26, 2011·Analytical Chemistry·Hyun Ju Yoo, Kristina Håkansson
Mar 3, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Todd W MitchellStephen J Blanksby

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