Analysis of urinary PSA glycosylation is not indicative of high-risk prostate cancer

Clinica Chimica Acta; International Journal of Clinical Chemistry
Sílvia BarrabésRosa Peracaula

Abstract

The levels of core fucosylation and α2,3-linked sialic acid in serum Prostate Specific Antigen (PSA), using the lectins Pholiota squarrosa lectin (PhoSL) and Sambucus nigra agglutinin (SNA), can discriminate between Benign Prostatic Hyperplasia (BPH) and indolent prostate cancer (PCa) from aggressive PCa. In the present work we evaluated whether these glycosylation determinants could also be altered in urinary PSA obtained after digital rectal examination (DRE) and could also be useful for diagnosis determinations. For this purpose, α2,6-sialic acid and α1,6-fucose levels of urinary PSA from 53 patients, 18 biopsy-negative and 35 PCa patients of different aggressiveness degree, were analyzed by sandwich ELLA (Enzyme Linked Lectin Assay) using PhoSL and SNA. Changes in the levels of specific glycosylation determinants, that in serum PSA samples were indicative of PCa aggressiveness, were not found in PSA from DRE urine samples. Although urine is a simpler matrix for analyzing PSA glycosylation compared to serum, an immunopurification step was necessary to specifically detect the glycans on the PSA molecule. Those specific glycosylation determinants on urinary PSA were however not useful to improve PCa diagnosis. This could be pr...Continue Reading

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Citations

Nov 20, 2018·Expert Review of Proteomics·Jan TkacPeter Kasak
Mar 26, 2019·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Robert LangMichael Vogeser
Mar 6, 2018·Analytical Chemistry·Guinevere S M KammeijerManfred Wuhrer

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