PMID: 12781484Jun 5, 2003Paper

Analysis of virulence plasmid gene expression of intra-macrophage and in vitro grown Rhodococcus equi ATCC 33701

Veterinary Microbiology
J Ren, J F Prescott

Abstract

Rhodococcus equi is a soil organism that infects macrophages of foals and immunocompromised humans. Virulence in foal isolates is tightly associated with an 80kb plasmid, which includes a pathogenicity island (PI) with a virulence-associated gene family, vap. A DNA microarray containing 66 of 69 putative open reading frames (ORFs) of the virulence plasmid was developed. Virulence plasmid gene expression of R. equi grown in macrophages or under different conditions in vitro was compared against in vitro growth at 30 degrees C, pH=7. When grown in macrophages, all seven vap family genes as well as six ORFs within, but not outside, the PI were induced. Cluster analysis of the gene expression matrix assembled from different growth conditions suggested that those genes that actively responded to environmental changes divided broadly into two groups. One group, orf1, 2, 5, 6-8, 12-15, 19, and 20 (which includes all the vap genes), was induced at 37 degrees C, mostly by low iron, and to a lesser extent by the synergy of low calcium and pH=5. The second group, orf3, 9, and 10, was induced at 37 degrees C by magnesium depletion (produced by EDTA treatment of growth medium). Temperature (37 degrees C) was the most important factor induci...Continue Reading

References

Jan 1, 1991·Reviews of Infectious Diseases·R L Harvey, J C Sunstrum
Aug 1, 1987·Veterinary Microbiology·J F Prescott
Jan 1, 1996·Trends in Microbiology·D M Mosser, M K Hondalus
Jun 16, 1997·Veterinary Microbiology·M K Hondalus
Mar 29, 2001·Journal of Bacteriology·C BoonT Dick
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·D R ShermanG K Schoolnik
Jul 17, 2001·Molecular Microbiology·E PérezC Martín

❮ Previous
Next ❯

Citations

Jul 28, 2006·Antonie van Leeuwenhoek·Marco Polidori, Albert Haas
Sep 3, 2013·Veterinary Microbiology·José A Vázquez-BolandAna Valero-Rello
Nov 19, 2011·Journal of Veterinary Internal Medicine·S GiguèreN M Slovis
Jul 9, 2004·Applied and Environmental Microbiology·Freddie H SharkeyRoger Marchant
May 15, 2007·Journal of Bacteriology·Gavin A ByrneWim G Meijer
Nov 19, 2003·American Journal of Veterinary Research·Misty C JordanRonald J Martens
Oct 22, 2011·The Veterinary Journal·Gary Muscatello
Mar 7, 2006·Journal of Veterinary Pharmacology and Therapeutics·J R HarringtonL R Bernstein
Dec 1, 2013·Equine Veterinary Journal. Supplement·J CrowleyG Muscatello
Oct 4, 2007·Equine Veterinary Journal·G MuscatelloJ A Vazquez-Boland
Jun 20, 2014·Equine Veterinary Journal·C GilesM D Barton
May 4, 2016·Veterinary Microbiology·Leticia T GresslerAgueda Castagna de Vargas
Jul 20, 2010·Veterinary Microbiology·Iain MacArthurJohn F Prescott
May 21, 2009·FEMS Microbiology Reviews·Kristine von Bargen, Albert Haas
Mar 24, 2016·PloS One·Carla GilesThiru Vanniasinkam
Jul 1, 2006·Critical Reviews in Microbiology·Sarita RanjanAkash Ranjan
Dec 13, 2017·Scientifica·Prashanth Chandramani-ShivalingappaBrett A Sponseller
Dec 4, 2019·Microbial Pathogenesis·Bibiana Petri da SilveiraAgueda Castagna de Vargas

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.