Abstract
Rhodococcus equi is a soil organism that infects macrophages of foals and immunocompromised humans. Virulence in foal isolates is tightly associated with an 80kb plasmid, which includes a pathogenicity island (PI) with a virulence-associated gene family, vap. A DNA microarray containing 66 of 69 putative open reading frames (ORFs) of the virulence plasmid was developed. Virulence plasmid gene expression of R. equi grown in macrophages or under different conditions in vitro was compared against in vitro growth at 30 degrees C, pH=7. When grown in macrophages, all seven vap family genes as well as six ORFs within, but not outside, the PI were induced. Cluster analysis of the gene expression matrix assembled from different growth conditions suggested that those genes that actively responded to environmental changes divided broadly into two groups. One group, orf1, 2, 5, 6-8, 12-15, 19, and 20 (which includes all the vap genes), was induced at 37 degrees C, mostly by low iron, and to a lesser extent by the synergy of low calcium and pH=5. The second group, orf3, 9, and 10, was induced at 37 degrees C by magnesium depletion (produced by EDTA treatment of growth medium). Temperature (37 degrees C) was the most important factor induci...Continue Reading
References
Jan 1, 1991·Microbiology and Immunology·K GotohI Yano
Jan 1, 1991·Reviews of Infectious Diseases·R L Harvey, J C Sunstrum
Aug 1, 1987·Veterinary Microbiology·J F Prescott
Jan 1, 1996·Trends in Microbiology·D M Mosser, M K Hondalus
Jan 1, 1996·Microbiology and Immunology·S TakaiS Tsubaki
Sep 1, 1996·Equine Veterinary Journal·J F PrescottG Perkins
Apr 1, 1997·Emerging Infectious Diseases·R Linder
Jun 16, 1997·Veterinary Microbiology·M K Hondalus
Jun 16, 1997·Veterinary Microbiology·S Giguère, J F Prescott
Aug 8, 1998·Microbiology·Ronald L SmithMichael E Maguire
Nov 18, 2000·Infection and Immunity·S TakaiJ F Prescott
Feb 7, 2001·Journal of Bacteriology·J M YarwoodP M Schlievert
Feb 13, 2001·Infection and Immunity·B A ByrneS A Hines
Mar 29, 2001·Journal of Bacteriology·C BoonT Dick
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Jun 8, 2001·Journal of Bacteriology·D Smajs, G M Weinstock
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·D R ShermanG K Schoolnik
Jul 12, 2001·Research in Microbiology·S BenoitA Hartke
Jul 17, 2001·Molecular Microbiology·E PérezC Martín
May 23, 2002·Bioinformatics·Wei Pan
Jun 18, 2002·Infection and Immunity·Stephanie BenoitAxel Hartke
Citations
Jul 28, 2006·Antonie van Leeuwenhoek·Marco Polidori, Albert Haas
Sep 3, 2013·Veterinary Microbiology·José A Vázquez-BolandAna Valero-Rello
Jul 18, 2006·Nucleic Acids Research·Sarita RanjanAkash Ranjan
Nov 19, 2011·Journal of Veterinary Internal Medicine·S GiguèreN M Slovis
Sep 8, 2006·Applied and Environmental Microbiology·G MuscatelloG F Browning
Jul 9, 2004·Applied and Environmental Microbiology·Freddie H SharkeyRoger Marchant
May 5, 2010·Infection and Immunity·Garry B CoulsonMary K Hondalus
Sep 24, 2005·Infection and Immunity·Daniel M WallWim G Meijer
May 15, 2007·Journal of Bacteriology·Gavin A ByrneWim G Meijer
Aug 20, 2004·Journal of Bacteriology·Dean A RussellWim G Meijer
Nov 19, 2003·American Journal of Veterinary Research·Misty C JordanRonald J Martens
Jun 9, 2005·American Journal of Veterinary Research·Jessica R HarringtonNoah D Cohen
Nov 1, 2011·The Veterinary Journal·Gary Muscatello
Oct 22, 2011·The Veterinary Journal·Gary Muscatello
Jul 7, 2009·Bioresource Technology·Liangwei DengLi Song
Mar 7, 2006·Journal of Veterinary Pharmacology and Therapeutics·J R HarringtonL R Bernstein
Dec 1, 2013·Equine Veterinary Journal. Supplement·J CrowleyG Muscatello
May 14, 2011·Microbiology·Raúl Miranda-CasoluengoWim G Meijer
Oct 4, 2007·Equine Veterinary Journal·G MuscatelloJ A Vazquez-Boland
Jun 20, 2014·Equine Veterinary Journal·C GilesM D Barton
Jun 23, 2015·Microbiology and Immunology·Tsutomu KakudaShinji Takai
May 4, 2016·Veterinary Microbiology·Leticia T GresslerAgueda Castagna de Vargas
Jul 20, 2010·Veterinary Microbiology·Iain MacArthurJohn F Prescott
May 21, 2009·FEMS Microbiology Reviews·Kristine von Bargen, Albert Haas
Jan 22, 2008·FEMS Microbiology Letters·Gavin A ByrneWim G Meijer
Aug 6, 2008·FEMS Microbiology Letters·Yang Hong, Mary K Hondalus
Oct 5, 2014·BMC Microbiology·Tsutomu KakudaShinji Takai
Oct 21, 2016·MicrobiologyOpen·Adam P RofePaul R Pryor
Mar 24, 2016·PloS One·Carla GilesThiru Vanniasinkam
Feb 16, 2007·Clinical and Vaccine Immunology : CVI·Stephanie JacksJohn F Prescott
Jul 1, 2006·Critical Reviews in Microbiology·Sarita RanjanAkash Ranjan
May 30, 2017·Frontiers in Microbiology·Marcel M TrevisaniSandro G Soares
Dec 13, 2017·Scientifica·Prashanth Chandramani-ShivalingappaBrett A Sponseller
Dec 4, 2019·Microbial Pathogenesis·Bibiana Petri da SilveiraAgueda Castagna de Vargas