Analysis of vitamin E metabolites including carboxychromanols and sulfated derivatives using LC/MS/MS.

Journal of Lipid Research
Qing JiangChao Yang

Abstract

Tocopherols and tocotrienols are metabolized via hydroxylation and oxidation of their hydrophobic side chain to generate 13'-hydroxychromanols (13'-OHs) and various carboxychromanols, which can be further metabolized by conjugation including sulfation. Recent studies indicate that long-chain carboxychromanols, especially 13'-carboxychromanol (13'-COOH), appear to be more bioactive than tocopherols in anti-inflammatory and anticancer actions. To understand the potential contribution of metabolites to vitamin E-mediated effects, an accurate assay is needed to evaluate bioavailability of these metabolites. Here we describe an LC/MS/MS assay for quantifying vitamin E metabolites using negative polarity ESI. This assay includes a reliable sample extraction procedure with efficacy of ≥ 89% and interday/intraday variation of 3-11% for major metabolites. To ensure accurate quantification, short-chain, long-chain, and sulfated carboxychromanols are included as external/internal standards. Using this assay, we observed that sulfated carboxychromanols are the primary metabolites in the plasma of rodents fed with γ-tocopherol or δ-tocopherol. Although plasma levels of 13'-COOHs and 13'-OHs are low, high concentrations of these compounds ar...Continue Reading

References

Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·W J WechterW H Wang
May 9, 2002·The Journal of Biological Chemistry·Timothy J Sontag, Robert S Parker
Sep 5, 2002·Free Radical Biology & Medicine·Simon A S PopeDavid P R Muller
Oct 9, 2002·The Journal of Nutrition·Marc BirringerRegina Brigelius-Flohé
May 6, 2003·Journal of Lipid Research·Hisako SaitoOsamu Igarashi
Feb 22, 2005·Journal of Lipid Research·Scott W LeonardMaret G Traber
Sep 9, 2005·Organic Letters·David J Maloney, Sidney M Hecht
Dec 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qing JiangJianjie Huang
Mar 13, 2010·Journal of Agricultural and Food Chemistry·Yang ZhaoChung S Yang
Aug 10, 2010·Free Radical Biology & Medicine·Marc BirringerMichael Ristow
Jun 6, 2012·The Journal of Biological Chemistry·Sabrina A BardowellRobert S Parker

❮ Previous
Next ❯

Citations

Nov 7, 2016·Free Radical Biology & Medicine·Francesco GalliNesrin Kartal Özer
Jan 16, 2018·Bioanalysis·Noemi Villaseca-GonzálezMaría Del Mar Arroyo-Jiménez
Nov 9, 2018·Frontiers in Neuroscience·Joaquin González-FuentesMaria M Arroyo-Jimenez
Sep 22, 2018·Nature Communications·Helmut PeinAndreas Koeberle
Jul 25, 2017·The Journal of Allergy and Clinical Immunology·Allison J BurbankMichelle L Hernandez
Apr 14, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Hadi HabibBenjamin C Moeller

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.