Analysis of wall antigens of Aspergillus fumigatus by two-dimensional immunoelectrophoresis

Journal of Medical Microbiology
V M Hearn, D W Mackenzie

Abstract

Water-soluble and surface-located antigens from Aspergillus fumigatus mycelium were analysed by two-dimensional electrophoresis (2D IEP) with selected sera. A complex pattern was seen when a water-soluble fraction reacted with hyperimmune sera. Significantly fewer precipitin peaks were seen when wall-located antigens were used in the 2D IEP system; this made possible the detection of a small number of recurring antigen-antibody interactions when sera from patients with aspergilloma or allergic bronchopulmonary aspergillosis were analysed. Many of these recurring precipitates were affected by lectins with specificity for alpha-D-glucose, alpha-D-mannose or N-acetyl-D-glucosamine. All the antigens were susceptible to hydrolysis by pronase, confirming the glycoprotein composition of many of these macromolecules.

Citations

Aug 1, 1986·European Journal of Clinical Microbiology·V Hopwood, D W Warnock
Jul 1, 1983·Journal of Biological Standardization·F C OddsP H Sneath
Feb 1, 1986·Lancet·A K TrullS Thiru
Jul 1, 1981·Histopathology·E N Saeed, R J Hay
May 1, 1992·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K E ReijulaJ N Fink
Jan 1, 1993·Clinical Microbiology Reviews·R F Hector

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