Analysis of wheat gene expression related to the oxidative stress response and signal transduction under short-term osmotic stress

Scientific Reports
Karolina DudziakMichał Nowak

Abstract

Water shortage is a major environmental stress that causes the generation of reactive oxygen species (ROS). The increase in ROS production induces molecular responses, which are key factors in determining the level of plant tolerance to stresses, including drought. The aim of this study was to determine the expression levels of genes encoding MAPKs (MAPK3 and MAPK6), antioxidant enzymes (CAT, APX and GPX) and enzymes involved in proline biosynthesis (P5CS and P5CR) in Triticum aestivum L. seedlings in response to short-term drought conditions. A series of wheat intervarietal substitution lines (ISCSLs) obtained by the substitution of single chromosomes from a drought-sensitive cultivar into the genetic background of a drought-tolerant cultivar was used. This source material allowed the chromosomal localization of the genetic elements involved in the response to the analyzed stress factor (drought). The results indicated that the initial plant response to drought stress resulted notably in changes in the expression of MAPK6 and CAT and both the P5CS and P5CR genes. Our results showed that the substitution of chromosomes 3B, 5A, 7B and 7D had the greatest impact on the expression level of all tested genes, which indicates that th...Continue Reading

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Citations

Jul 19, 2020·Functional & Integrative Genomics·Shahidul IslamRudi Appels
Dec 31, 2019·Oxidative Medicine and Cellular Longevity·Yuhan DongShenchun Qu
Jun 30, 2019·International Journal of Molecular Sciences·Ahmed SallamAndreas Börner
Oct 18, 2020·Molecular Biology Reports·Rakesh KumarGyanendra Pratap Singh
Feb 27, 2021·Frontiers in Plant Science·Mahmoud GadMaoteng Li
Mar 7, 2021·International Journal of Molecular Sciences·Judit BányaiGyula Vida

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Datasets Mentioned

BETA
AF079318
AY173962
CJ705892

Methods Mentioned

BETA
transgenic
proteomic profiling
MDA
electrophoresis
PCR

Software Mentioned

Coffee
PrimerBLAST
geNorm
NormFinder
Statistica
BestKeeper
RefGenes
BLAST
RefFinder

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