Analyte-driven self-assembly of graphene oxide sheets onto hydroxycamptothecin-functionalized upconversion nanoparticles for the determination of type I topoisomerases in cell extracts

Analytical and Bioanalytical Chemistry
Xu Wang, Xiu-Ping Yan

Abstract

Type I topoisomerases (TOPOI), a potential diagnostic biomarker and a target for chemotherapeutic agents, play essential roles in DNA replication, transcription, chromosome segregation, and recombination. It is essential to develop analytical methods for accurate detection of TOPOI in biological fluids for early diagnosis of diseases. Here we show an assay for TOPOI on the basis of the target-induced self-assembly of graphene oxide (GO) sheets onto hydroxycamptothecin-functionalized upconversion nanoparticles (HCPT-UCNPs). The dipole-dipole coupling of HCPT-UCNPs (donor) and GO (acceptor) regulated by TOPOI enables Förster resonance energy transfer between the donor and the acceptor. Integration of minimal autofluorescence and highly specific affinity into the developed nanosensor allows reliable detection of TOPOI in the nanomolar range with the detection limit of 0.29 nM. The detection of TOPOI in breast cancer cells with recoveries from 96.3 to 103.7% shows the availability of the proposed assay in complicated samples. Graphical abstract ᅟ.

References

Feb 17, 1993·Journal of the National Cancer Institute·W J SlichenmyerS H Kaufmann
Jun 8, 2001·Annual Review of Biochemistry·J J Champoux
Jun 4, 2002·Nature Reviews. Molecular Cell Biology·James C Wang
Jun 9, 2005·Natural Product Research·Dharmarajan SriramTanushree Ratan Bal
Sep 23, 2006·Nature Reviews. Cancer·Yves Pommier
Jan 8, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Marcin Skrzypski
Sep 11, 2008·Expert Opinion on Therapeutic Targets·Giovanni Luca BerettaFranco Zunino
Nov 28, 2008·Journal of the American Chemical Society·Fung-Ming Siu, Chi-Ming Che
May 16, 2009·Expert Opinion on Therapeutic Patents·Serena Basili, Stefano Moro
Nov 26, 2010·Nature Chemistry·Kian Ping LohManish Chhowalla
Sep 7, 2011·Journal of the American Chemical Society·Jinliang LiuFuyou Li
Mar 27, 2013·Sensors·Lærke Bay MarcussenBirgitta Ruth Knudsen
Jan 28, 2015·Journal of the American Chemical Society·Juanjuan PengYoung-Tae Chang
Mar 24, 2016·Analytical and Bioanalytical Chemistry·Xiaofei GuoZhouping Wang
Jul 9, 2017·Analytical and Bioanalytical Chemistry·Hans H Gorris, Ute Resch-Genger
Oct 5, 2017·Analytical and Bioanalytical Chemistry·Xiude HuaMinghua Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.