PMID: 2490524Jan 1, 1989Paper

Analytical applications of enhanced drug luminescence

Journal of Pharmaceutical and Biomedical Analysis
W R Baeyens, B L Ling

Abstract

Luminescence emission from drugs is strongly dependent on their physicochemical environment. Several biomedically and environmentally important compounds and pharmaceuticals exhibit sufficient intrinsic luminescence properties to allow their determination by high-performance liquid chromatography (HPLC) with fluorimetric, chemiluminescence or room temperature phosphorimetric detection. In the case of weakly fluorescing compounds it is possible to use the dependence of the emitted radiation on the molecular environment at the moment of measurement. The composition of the eluent, i.e. solvents, added salts and buffers, pH and ionic strength, oxygen content and temperature, are of the highest importance for the luminescence detection of drugs in solution (e.g. in liquid chromatography) or adsorbed onto solid surfaces (e.g. in thin-layer chromatography). Post-column or post-plate acid-base manipulation and the use of specific reagents may remarkably enhance the observed luminescence of several molecules. The term "enhancement" of luminescence comprises various sample treatments leading to an increase of the emitted radiation. These treatments include the addition of non-fluorescent compounds to, or the creation of organized media (...Continue Reading

References

Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·B L LingP De Moerloose
Jul 11, 1986·Journal of Chromatography·K M MarnelaH Vapaatalo
Aug 9, 1985·Journal of Chromatography·M Arunyanart, L J Cline Love
Jan 1, 1988·Journal of Pharmaceutical and Biomedical Analysis·S El GezawiJ H Perrin
Jan 1, 1988·Journal of Pharmaceutical and Biomedical Analysis·S El GezawiJ H Perrin
Jan 1, 1986·Journal of Pharmaceutical and Biomedical Analysis·G Guilbault

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