Analytical approach to determining human biogenic amines and their metabolites using eVol microextraction in packed syringe coupled to liquid chromatography mass spectrometry method with hydrophilic interaction chromatography column

Talanta
Lucyna KoniecznaTomasz Bączek

Abstract

Analysis of biogenic amines (BAs) in different human samples provides insight into the mechanisms of various biological processes, including pathological conditions, and thus may be very important in diagnosing and monitoring several neurological disorders and cancerous tumors. In this work, we developed a simple and fast procedure using a digitally controlled microextraction in packed syringe (MEPS) coupled to liquid chromatography mass spectrometry (LC-MS) method for simultaneous determination of biogenic amines, their precursors and metabolites in human plasma and urine samples. The separation of 12 low molecular weight and hydrophilic molecules with a wide range of polarities was achieved with hydrophilic interaction chromatography (HILIC) column without derivatization step in 12 min. MEPS was implemented using the APS sorbent in semi-automated analytical syringe (eVol(®)) and small volume of urine and plasma samples, 5 0µL and 100 μL, respectively. We evaluated important parameters influencing MEPS efficiency, including stationary phase selection, sample pH and volume, number of extraction cycles, and washing and elution volumes. In optimized MEPS conditions, the analytes were eluted by 3 × 50 μL of methanol with 0.1% form...Continue Reading

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Citations

Nov 12, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Liu YangMohamed Abdel-Rehim
Sep 6, 2019·Critical Reviews in Analytical Chemistry·Waqas AhmadM S El-Shahawi
May 30, 2020·Journal of Pharmaceutical and Biomedical Analysis·Ana Sofia Abrantes DiasBruno Manadas

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