Analytical cell adhesion chromatography reveals impaired persistence of metastatic cell rolling adhesion to P-selectin

Journal of Cell Science
Jaeho OhSusan N Thomas

Abstract

Selectins facilitate the recruitment of circulating cells from the bloodstream by mediating rolling adhesion, which initiates the cell-cell signaling that directs extravasation into surrounding tissues. To measure the relative efficiency of cell adhesion in shear flow for in vitro drug screening, we designed and implemented a microfluidic-based analytical cell adhesion chromatography system. The juxtaposition of instantaneous rolling velocities with elution times revealed that human metastatic cancer cells, but not human leukocytes, had a reduced capacity to sustain rolling adhesion with P-selectin. We define a new parameter, termed adhesion persistence, which is conceptually similar to migration persistence in the context of chemotaxis, but instead describes the capacity of cells to resist the influence of shear flow and sustain rolling interactions with an adhesive substrate that might modulate the probability of extravasation. Among cell types assayed, adhesion persistence to P-selectin was specifically reduced in metastatic but not leukocyte-like cells in response to a low dose of heparin. In conclusion, we demonstrate this as an effective methodology to identify selectin adhesion antagonist doses that modulate homing cell ...Continue Reading

References

Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·P S FrenetteD D Wagner
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y J KimA Varki
Sep 16, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S AignerK Ley
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·J FritzD Anselmetti
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S D RobinsonR O Hynes
Jun 6, 2000·Advanced Drug Delivery Reviews·K KonstantopoulosL V McIntire
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·S Chen, T A Springer
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·L BorsigA Varki
Mar 20, 2001·Biotechnology and Bioengineering·A W Greenberg, D A Hammer
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lubor BorsigAjit Varki
Aug 15, 2002·The Journal of Cell Biology·Tadayuki YagoRodger P McEver
Jan 11, 2003·The Journal of Biological Chemistry·William HanleyKonstantinos Konstantopoulos
May 9, 2003·Nature·Bryan T MarshallCheng Zhu
Jul 31, 2003·Thrombosis and Haemostasis·Jian-Guo Wang, Jian-Guo Geng
Apr 16, 2004·American Journal of Physiology. Cell Physiology·Monica M Burdick, Konstantinos Konstantopoulos
May 26, 2004·Journal of Cell Science·William D HanleyKonstantinos Konstantopoulos
Dec 15, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·William D HanleyKonstantinos Konstantopoulos
Jul 15, 2006·Journal of Thrombosis and Haemostasis : JTH·H van GenderenC P M Reutelingsperger
Feb 1, 2008·American Journal of Physiology. Cell Physiology·Christina S AlvesKonstantinos Konstantopoulos
Apr 1, 2008·The Journal of Biological Chemistry·Susan N ThomasKonstantinos Konstantopoulos
May 6, 2009·Annual Review of Biomedical Engineering·Konstantinos Konstantopoulos, Susan N Thomas
May 12, 2011·Journal of Cell Science·Phrabha S RamanKonstantinos Konstantopoulos
Jan 27, 2012·Annals of the New York Academy of Sciences·Suhair Hanna, Amos Etzioni
Mar 15, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Matthew R DallasKonstantinos Konstantopoulos
Apr 17, 2012·International Journal of Cell Biology·Gerd Bendas, Lubor Borsig
May 10, 2013·PloS One·Frank A W CoumansLeon W M M Terstappen

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Citations

Mar 2, 2016·Annual Review of Biomedical Engineering·Susan N ThomasErin E Edwards
Mar 7, 2017·Integrative Biology : Quantitative Biosciences From Nano to Macro·Erin Elizabeth Edwards, Susan Napier Thomas
Nov 12, 2018·Cell Systems·Erin Elizabeth EdwardsSusan Napier Thomas
May 2, 2021·Biophysical Journal·Adam B YasunagaIsaac T S Li

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