Abstract
Two modern, fundamentally different methods were used for a detailed investigation of enantioselective drug-protein interactions, a surface plasmon resonance (SPR)-based Biacore 2000 biosensor assay and the previously validated HPLC perturbation method (HPLC-PM). This is the first time SPR has been used for this purpose. The fundamental features of the two methods were investigated, and the consequences for operation and data evaluation were addressed. With HPLC-PM, chiral data could be obtained directly from the racemic mixture, whereas a separate analysis of each pure enantiomer was required to obtain chiral data with SPR. It was shown that if chirality is not attributed in the SPR analysis, misleading average racemic binding constants will be obtained. Both drug and protein consumption were considerably higher with HPLC-PM. HPLC-PM was found to be best suited for measurements of weak affinity interactions, whereas the SPR method was best for strong interactions. With both methods, the presence of DMSO in the samples severely affected the interactions, introducing errors. The binding of the beta-blockers alprenolol and propranolol to Cel7a cellulase was used as a model system. These methods gave results that agreed quite well...Continue Reading
References
Dec 15, 1991·Journal of Immunological Methods·R KarlssonL Mattsson
Jul 22, 1994·Science·C DivneT A Jones
Jul 29, 1996·FEBS Letters·H HenrikssonG Pettersson
Apr 20, 1999·Nature Biotechnology·O HofstetterB S Green
Oct 28, 1999·Journal of Chromatography. a·H HenrikssonG Johansson
May 23, 2000·Journal of Medicinal Chemistry·A Frostell-KarlssonR Karlsson
Dec 15, 2000·Journal of Molecular Biology·J StåhlbergT A Jones
Nov 28, 2001·Analytical Chemistry·T Fornstedt, G Guiochon
Oct 3, 2002·Nature Reviews. Drug Discovery·Israel AgranatJohn Caldwell
Feb 27, 2003·Biosensors & Bioelectronics·Ateeq AhmadAlan P Breau
Apr 22, 2003·Journal of Chromatography. a·Patrik ForsénTorgny Fornstedt
Feb 12, 2004·Journal of Molecular Recognition : JMR·Roberto CorradiniRoberto Gambari
May 12, 2004·Journal of Molecular Recognition : JMR·Robert Karlsson
May 26, 2004·Analytical Biochemistry·David G Myszka
Citations
Sep 24, 2013·Analytical Biochemistry·Geraldine Cilpa-KarhuMarja-Liisa Riekkola
May 4, 2013·Journal of Chromatography. a·Yuping ZhangDeshan Feng
Aug 10, 2013·Journal of Chromatography. a·Víctor Agmo HernándezTorgny Fornstedt
Jan 1, 2010·Sensors·Katsuhiko ArigaJonathan P Hill
May 3, 2014·Analytical and Bioanalytical Chemistry·Katriina LipponenMarja-Liisa Riekkola
Apr 13, 2013·Biosensors & Bioelectronics·Longhua GuoGuonan Chen
Dec 3, 2009·Proteomics·Chi Bun ChingWei Ning Chen
Feb 26, 2008·Analytical Biochemistry·Rangan MallikDavid S Hage
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Apr 4, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bo ZhouYi Liu
May 23, 2009·Molecular BioSystems·Jianjun SuiWei Ning Chen
Oct 26, 2010·Electrophoresis·Ereny F MorcosDarryl Bornhop
May 28, 2009·Journal of Separation Science·Jörgen SamuelssonTorgny Fornstedt
Jun 4, 2014·Chemistry : a European Journal·Alessia CiogliAlberto Cavazzini
Mar 4, 2020·Journal of Separation Science·Laurence MuhrLaetitia Canabady-Rochelle
Jul 28, 2012·Lab on a Chip·Longhua GuoGuonan Chen
Nov 17, 2020·Oncotarget·Myoung Sook KimAntonino Passaniti
Apr 3, 2009·Analytical Chemistry·Peter SandbladTorgny Fornstedt
Jul 28, 2009·Analytical Chemistry·Alberto CavazziniFilippo Bedani
Apr 8, 2021·Analytical Chemistry·Yingqian WangHaibing Li
May 15, 2021·Optics Express·Aneeth KakkanattuFrank Vollmer
Jul 24, 2012·Analytical Chemistry·Nicola MarchettiAlberto Cavazzini
Dec 12, 2012·Analytical Chemistry·Alberto CavazziniFrancesco Gasparrini
Jun 7, 2015·Analytical Chemistry·Martina CataniAlberto Cavazzini