Analytical characterization of microfabricated SU-8 emitters for electrospray ionization mass spectrometry

Journal of Mass Spectrometry : JMS
Tiina SikanenTapio Kotiaho

Abstract

We present a detailed optimization and characterization of the analytical performance of SU-8-based emitters for electrospray ionization mass spectrometry (ESI/MS). The improved SU-8 fabrication process presented here enhances patterning accuracy and reduces the time and cost of fabrication. All emitters are freestanding and enable sample delivery by both pressure-driven and spontaneous flows. The optimized emitter design incorporates a sharp, double-cantilevered tip implemented to the outlet of an SU-8 microchannel and provides highly sensitive ESI/MS detection. Moreover, the optimized design allows the use of relatively large microchannel dimensions (up to 200 x 50 microm(2), w x h) without sacrificing the detection sensitivity. This is advantageous with a view of preventing emitter clogging and enabling reproducible analysis. The measured limits of detection for the optimized emitter design were 1 nM for verapamil and 4 nM for Glu-fibrinopeptide B with good quantitative linearities between 1 nM and 10 microM (R(2) = 0.9998) for verapamil and between 4 nM and 3 microM (R(2) = 0.9992) for Glu-fibrinopeptide B. The measured tip-to-tip repeatability for signal intensity was 14% relative standard deviation (RSD) (n = 3; 5 microM ...Continue Reading

References

Jan 1, 1996·Analytical Chemistry·M Wilm, M Mann
Feb 1, 1997·Analytical Chemistry·Q XueB L Karger
Feb 5, 2000·Analytical Chemistry·L LickliderT D Lee
Aug 30, 2000·Rapid Communications in Mass Spectrometry : RCM·E X VrouweJ van der Greef
Sep 20, 2000·Analytical Chemistry·G A SchultzS Zhang
May 17, 2001·Analytical Chemistry·J KameokaJ Henion
Aug 28, 2001·Journal of Chromatography. a·J S Kim, D R Knapp
Jan 31, 2002·Analytical Chemistry·T C RohnerH H Girault
Aug 8, 2002·Proteomics·Véronique GobryHubert H Girault
Dec 5, 2002·Analytical Chemistry·Jun KameokaH G Craighead
Feb 6, 2003·Rapid Communications in Mass Spectrometry : RCM·Johan SjödahlJohan Roeraade
Oct 2, 2003·Journal of Chromatography. a·Yuji TachibanaShin Nakamura
Nov 13, 2003·Electrophoresis·Iulia M LazarBarry L Karger
Apr 15, 2004·Analytical Chemistry·Solomon SsenyangeMark T McDermott
May 26, 2004·Lab on a Chip·M SchillingR Hergenroder
Jul 9, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Séverine Le GacChristian Rolando
Jul 23, 2004·Lab on a Chip·Malin SvedbergFredrik Nikolajeff
Nov 2, 2004·Analytical Chemistry·Terry KoernerRichard D Oleschuk
Jul 20, 2005·Lab on a Chip·Tiina SikanenTapio Kotiaho
Aug 11, 2005·Analytical and Bioanalytical Chemistry·Yan-Zin ChangGou-Jen Wang
Nov 12, 2005·Electrophoresis·Santeri TuomikoskiSami Franssila
Sep 16, 2006·Electrophoresis·Maria AgirregabiriaJesús M Ruano-López
Jul 20, 2007·Analytical Chemistry·Tiina SikanenTapio Kotiaho
Oct 25, 2007·Rapid Communications in Mass Spectrometry : RCM·Teemu NissiläRaimo A Ketola

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Citations

Nov 13, 2010·Rapid Communications in Mass Spectrometry : RCM·Andrea E KirbyAaron R Wheeler
Jun 11, 2009·Mass Spectrometry Reviews·Tiina SikanenRaimo A Ketola
Jun 30, 2012·Analytical Chemistry·Sung Hwan YoonDavid R Goodlett
Jul 17, 2014·Lab on a Chip·Steve Arscott
Dec 24, 2018·Micromachines·Anand TatikondaSami Franssila
Jul 29, 2009·The Journal of Physical Chemistry. B·Susan D GillmorPaul S Weiss

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