Analytical cytometric approaches to heterogeneous cell populations in solid tumors: a review

Cytometry
L A DethlefsenR M Riley

Abstract

The problems encountered in studying the heterogeneity of cells in solid tumors is reviewed with emphasis on the role of various analytical cytometric assays for studying both the biology and the dynamics and proliferating, quiescent and dead malignant cells in vitro and in vivo. Due to advances in cytometric technology, many interesting in vitro studies on tumor cells heterogeneity have been and will be conducted over the next several years. For example, the acidic acridine orange staining of HeLa cells in suspension culture does readily discriminate between proliferating and quiescent cells. Some of these assays have been and others will be extended to in vivo studies. However, it is obvious that either the current analytical cytometric techniques must be modified and refined to permit better resolution for the complex situation in vivo or other new analytical cytometric techniques will have to be developed before many interesting studies on tumor cell heterogeneity in vivo can be addressed with reasonable efficiency.

References

Jun 1, 1975·Experimental Cell Research·R A Tobey, H A Crissman
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·M V Pimm, R W Baldwin
Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Z DarzynkiewiczM R Melamed
Jul 1, 1979·Journal of Cellular Physiology·C P StannersJ W Pollard
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
Mar 1, 1973·Cell and Tissue Kinetics·L A Dethlefsen, R M Riley
Oct 1, 1968·Biophysical Journal·S I Rubinow
Jun 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K D Bauer, L A Dethlefsen

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Citations

Feb 1, 1985·Developmental Biology·T M SzopaG M Happ
Jan 1, 1984·Pharmacology & Therapeutics·S C Barranco
Nov 26, 1999·International Journal of Radiation Oncology, Biology, Physics·F ZölzerH Sack
Feb 28, 2013·Expert Opinion on Drug Delivery·Abraham H Abouzeid, Vladimir P Torchilin
Jan 1, 1984·Cancer Investigation·F Traganos
Jan 1, 1993·International Journal of Radiation Biology·F ZölzerT Pelzer
Nov 1, 1985·The Journal of Urology·A J Hough

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