Analytical development of electrospray and nanoelectrospray mass spectrometry in combination with liquid chromatography for the characterization of proteins

Journal of Chromatography. B, Biomedical Sciences and Applications
T A FliggeM Przybylski

Abstract

Mass spectrometry has significantly extended its applicability in the area of characterization of protein structures. Electrospray ionization enables on-line coupling with liquid chromatography which has become a powerful tool for the characterization of peptide and protein mixtures. The most recent development of a nanoelectrospray source, using capillary forces for a particularly mild analyte transport and ionization into the mass spectrometer, opens a wide field for applications to protein structure analysis. In this paper, the analytical development of liquid chromatography-electrospray ionization mass spectrometry and nano-electrospray ionization mass spectrometry, adapted to an electrospray ionization quadrupole mass spectrometer and its application to the characterization of noncovalent protein complexes are described.

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Citations

Feb 2, 1999·Journal of the American Society for Mass Spectrometry·T A FliggeM Przybylski
Aug 17, 2001·Rapid Communications in Mass Spectrometry : RCM·C FierensA P De Leenheer
Aug 28, 2001·Journal of Mass Spectrometry : JMS·R GrandoriN Müller
Sep 13, 2011·Clinics in Laboratory Medicine·Dominik DomanskiChristoph H Borchers
Oct 12, 2004·The International Journal of Biochemistry & Cell Biology·Soghra Khatun HaqRizwan Hasan Khan
Jan 9, 2007·Rapid Communications in Mass Spectrometry : RCM·Anusak SirikatithamTsutomu Masujima
Aug 5, 2000·Rapid Communications in Mass Spectrometry : RCM·G G KuhnleU Hahn
Dec 10, 1999·Journal of Mass Spectrometry : JMS·P T SzabóT Janáky
Dec 21, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A Vlahou, M Fountoulakis
Aug 24, 2013·Analytical Chemistry·Elizabeth M YuillLane A Baker
Nov 20, 2001·Chemical Reviews·K B Tomer

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