PMID: 568943Nov 1, 1978

Analytical fractionation of cultured hepatoma cells (HTC cells)

Biochimica Et Biophysica Acta
P Lopez-SauraP Tulkens


Homogenates of HTC cells have been fractionated by differential centrifugation (in four particulate fractions: N, M, L, P, and a supernatant S) or isopycnic banding in linear sucrose gradients. On this basis, the following subcellular organelles may be characterized: (i) Mitochondria, detected by cytochrome oxidase and succinodehydrogenase, are collected in the M and L fractions, and equilibrate, as a narrow band, at a median buoyant density of 1.18 g/cm3. (ii) Lysosomes, detected by the latent hydrolases beta-glycerophosphatase and N-acetyl-beta-glucosaminidase, are largely sedimented in the M and L fractions, and display a broad density distribution pattern with a median value of 1.17 g/cm3. This density is decreased or increased after cultivation of the cells in presence of Triton WR-1339 or Dextran 500, respectively. The behavior of cathepsin D is somewhat at variance with that of the two other hydrolases. (iii) Plasma membrane is tentatively detected by alkaline phosphodiesterase I. Largely recovered in the P fraction, this enzyme equilibrates at a median density close to that of the lysosomal hydrolases; the bulk of cholesterol and about half of the leucyl-2-naphthylamidase are closely associated with alkaline phosphodies...Continue Reading


Jan 1, 1988·The International Journal of Biochemistry·S M MahlerB C Shanley
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G G RousseauD K Granner
Apr 1, 1983·The Journal of Experimental Medicine·C Darte, H Beaufay
Oct 1, 1979·Journal of Ultrastructure Research·M A Leroy-HouyetP Baudhuin
Mar 1, 1981·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J C BlanchardA Trouet


Jan 1, 1976·Journal of Supramolecular Structure·J TwetoD Doyle
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J Bartholeyns, P Baudhuin
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·E B ThompsonJ F Curran
Dec 19, 1969·Annals of the New York Academy of Sciences·P Baudhuin
Jan 1, 1964·Journal of Theoretical Biology·C De Duve

Related Concepts

Cytochrome C Oxidase
Set of Polysomal Ribosomes
Liver Carcinoma
Phosphodiesterase I
Hepatoma, Novikoff
Triton WR-1339

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