Analytical methods for the characterization and quality control of pharmaceutical peptides and proteins, using erythropoietin as an example

Pharmaceutica acta Helvetiae
D GilgM F Zimmermann

Abstract

In the last twenty years many peptides and proteins were introduced to the market for pharmaceutical use. The high complexity of the biomacromolecules requires the use not only of physicochemical methodologies but also of immunochemical and biological techniques for the characterization and quality control of these substances. Characterization of the active conformation and post-translational modifications is important for the understanding of the in vivo activity of peptides and proteins. Among other spectroscopic techniques circular dichroism has become a helpful tool for evaluating the secondary and tertiary structure of proteins and peptides. A combination of several analytical methods is necessary for a comprehensive characterization of the substance under investigation and for an appropriate quality control of the product concerning identity, purity and potency. Suitable analytical methodologies are discussed using erythropoietin as an example.

References

Aug 1, 1975·Endocrinology·E GoldwasserD Sikkema
Jan 1, 1992·Annual Review of Biochemistry·J Rizo, L M Gierasch
Mar 10, 1990·European Journal of Biochemistry·E TsudaR Sasaki
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M TakeuchiA Kobata
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F K LinZ Stabinsky
Aug 11, 1980·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K Murray
Jan 1, 1994·Faraday Discussions·P XieM Diem
Jan 1, 1995·European Journal of Clinical Pharmacology·S Vozeh
Apr 15, 1995·Analytical Chemistry·R S RushV Katta
Jan 14, 1994·Journal of Chromatography. a·P Ng, G Mitra
Nov 1, 1995·Journal of Pharmaceutical Sciences·A M DePaolisB G Sharma

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Citations

Dec 11, 2014·Therapeutic Delivery·Ravi Vaishya, Ashim K Mitra
Sep 25, 2014·Expert Opinion on Drug Delivery·Ravi VaishyaAshim K Mitra
May 24, 2006·Electrophoresis·Michael J LittlePieter K Roos

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