Analytical performance of an immunoassay to measure proenkephalin

Clinical Biochemistry
Leslie J DonatoAllan S Jaffe

Abstract

Endogenous opioids, enkephalins, are known to increase with acute kidney injury. Since the mature pentapeptides are unstable, we evaluated the performance of an assay that measures proenkephalin 119-159 (PENK), a stable peptide formed concomitantly with mature enkephalins. PENK assay performance was evaluated on two microtiterplate/chemiluminescence sandwich immunoassay formats that required 18 or 1 h incubation times. PENK concentration was measured in plasma from healthy individuals to establish a reference interval and in patients with varied levels of kidney function and comorbidities to assess the association with measured glomerular filtration rate (mGFR) using iothalamate clearance. Assay performance characteristics in plasma were similar between the assay formats. Limit of quantitation was 26.0 pmol/L (CV = 20%) for the 1 h assay and 17.3 pmol/L (CV = 3%) for the 18 h assay. Measurable ranges were 26-1540 pmol/L (1 h assay) and 18-2300 pmol/L (18 h assay). PENK concentrations are stable in plasma stored ambient to 10 days, refrigerated to at least 15 days, and frozen to at least 90 days. Results were comparable in paired SST serum and EDTA plasma. Age and sex were not associated with PENK concentrations in healthy indiv...Continue Reading

Citations

May 17, 2019·Circulation. Heart Failure·Johanna E EmmensAdriaan A Voors
Jul 1, 2020·Clinical Chemistry and Laboratory Medicine : CCLM·Stan J F HartmanSaskia N de Wildt
Jun 17, 2020·Annals of Laboratory Medicine·Hanah KimSalvatore Di Somma
Jun 24, 2020·Peptides·Richard J Bodnar
Jul 20, 2021·Intensive Care Medicine Experimental·Attila FrigyesiHans Friberg
Jan 29, 2020·Burns : Journal of the International Society for Burn Injuries·François DépretUNKNOWN PRONOBURN group

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