Analytical performance of the serum free light chain assay

Clinical Chemistry and Laboratory Medicine : CCLM
Pierre-Yves BriandLucienne Guenet

Abstract

The Freelite system for nephelometric or turbidimetric measurement of serum free light chains (FLCs) has been available since 2001. It has been valuable for the management of patients with oligosecretory myeloma, light chain myeloma and AL amyloidosis. However, there are several limitations of the method. The goal of this study was to evaluate the analytical performance of the FLC assay. Titrated controls and clinical serum specimens were used to determine precision and post-dilution recovery. As reported elsewhere, we found that the assay had several limitations, including poor post-dilution linearity and overestimation by nephelometry. These data demonstrate that the results of the FLC assay must be interpreted jointly by the clinician and the biologist, taking into account the individual patient's clinical and biological characteristics.

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Citations

Jun 15, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Stanley S Levinson
Feb 16, 2013·F1000 Medicine Reports·Judith A Brebner, Robert A Stockley
Feb 7, 2013·Clinical Biochemistry·Sara AltinierMario Plebani
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Jun 15, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Henk Te VelthuisRoel Melsert
Aug 26, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Stanley S Levinson
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Feb 10, 2011·Clinical Chemistry·Stanley S Levinson
Oct 16, 2019·Critical Reviews in Clinical Laboratory Sciences·Laura CaponiMaria Franzini
Jan 11, 2014·Clinical Chemistry and Laboratory Medicine : CCLM·Joannes F M JacobsHenk Te Velthuis
Dec 7, 2013·Expert Review of Molecular Diagnostics·Maria Stella Graziani, Giampaolo Merlini
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Mar 5, 2021·Clinical Biochemistry·Luis J Morales-García, María S Pacheco-Delgado
Feb 4, 2014·Annals of Clinical Biochemistry·Malini V BholeKarthik Ramasamy

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