Analytical performances of a DNA-ligand system using time-resolved fluorescence for the determination of ochratoxin A in wheat

Analytical and Bioanalytical Chemistry
Annalisa De GirolamoAngelo Visconti

Abstract

The analytical performances of a novel DNA-ligand system using the time-resolved fluorescence (TRF) response of ochratoxin A (OTA)-terbium-DNA aptamer interaction were tested for the quantitative determination of OTA in wheat. Wheat was extracted with acetonitrile/water (60:40, v/v) followed by clean-up through affinity columns containing a DNA-aptamer-based oligosorbent. Then, OTA was detected by TRF spectroscopy after reaction with a terbium fluorescent solution containing the DNA-aptamer probe. The entire procedure was performed in less than 30 min, including sample preparation, and allowed analysis of several samples simultaneously with a 96-well microplate reader. The average recovery from samples spiked with 2.5-25 μg kg(-1) OTA was 77%, with a relative standard deviation lower than 6% and a quantification limit of 0.5 μg kg(-1). Comparative analyses of 29 naturally contaminated (up to 14 μg kg(-1)) wheat samples using the aptamer-affinity column/TRF method or the immunoaffinity column/high-performance liquid chromatography method showed good correlation (r = 0.985) in the range tested. The trueness of the aptamer-based method was additionally assessed by analysis of two quality control wheat materials for OTA. The DNA-li...Continue Reading

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Citations

Jun 4, 2013·Analytical and Bioanalytical Chemistry·Qiang ZhaoHailin Wang
Nov 8, 2013·Toxins·Amina RhouatiJean-Louis Marty
Nov 15, 2012·Journal of Nucleic Acids·Maureen McKeague, Maria C Derosa
May 15, 2013·Journal of Pharmaceutical and Biomedical Analysis·Tina ŠmucHenning Ulrich
Jan 9, 2016·Critical Reviews in Food Science and Nutrition·J Fernando Huertas-PérezLaura Gámiz-Gracia
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Jul 23, 2015·BioMed Research International·Ka Lok Hong, Letha J Sooter
Oct 8, 2014·Analytical and Bioanalytical Chemistry·Valérie PichonAudrey Combès
Oct 23, 2014·Applied Biochemistry and Biotechnology·Xiaofei Liu, Xuewu Zhang
Jun 24, 2016·Analytical and Bioanalytical Chemistry·Xiaodong GuoJiaqi Wang
Feb 22, 2017·Scientific Reports·Krisztina PerczeTamás Mészáros
Dec 23, 2015·Toxins·Tai Hwan Ha
Apr 6, 2019·Mycotoxin Research·Md Zakir Hossain, Chris M Maragos

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