Analytical performances of FT-IR spectrometry and imaging for concentration measurements within biological fluids, cells, and tissues

The Analyst
Cyril PetiboisGérard Déléris

Abstract

FT-IR spectrometry has proved to be a useful tool for determining a series of plasma molecular concentrations. Dedicated experiments were first performed to test the analytical performance that could be obtained by FT-IR spectrometry using a synthesized N3-peptide exhibiting a -N3 absorption centered at 2110 cm(-1), a spectral region where no organic material of biological samples absorbs. Further, we investigated whether this technology was able to allow quantification of metabolic parameters (glucose and lactic acid) within plasma, cells, and tissues as an alternative method to the "classical" biochemical approaches, which require sophisticated biological material treatment and expensive reagents. For this purpose we used a series of plasma samples to determine glucose and lactic acid concentrations, which are common markers of cancer growth. We compared the results of the main spectral data treatments commonly achieved for FT-IR data analysis, such as univariate (Beer-Lambert) or multivariate (PLS) calibrations, as well as the deconvolution of the spectral interval of interest (1200-900 cm(-1)). No significant differences were found regarding the analytical performances of these methods. Spectral deconvolution was finally un...Continue Reading

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Citations

Jun 4, 2008·Analytical and Bioanalytical Chemistry·Cyril Petibois, Mariangela Cestelli Guidi
Jul 19, 2008·Analytical and Bioanalytical Chemistry·Katia WehbeCyril Petibois
Jun 3, 2010·Analytical and Bioanalytical Chemistry·C PetiboisA Marcelli
Dec 1, 2010·Bulletin of Experimental Biology and Medicine·S A KhaustovaA G Tonevitsky
Dec 24, 2009·Journal of Synchrotron Radiation·Cyril PetiboisAugusto Marcelli
Aug 25, 2009·Applied and Environmental Microbiology·Crystal A VargasMark R Riley
Feb 1, 2014·Analytical and Bioanalytical Chemistry·Adrian TravoIsabelle Forfar
Oct 26, 2013·Biochimica Et Biophysica Acta·Allison DerenneErik Goormaghtigh
Oct 15, 2011·Journal of Pharmaceutical and Biomedical Analysis·P V AjayakumarA Samad
Oct 7, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Paola GrimaldiAgostina Congiu Castellano
Apr 22, 2009·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Renming LiuMinzhen Si
Oct 28, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Isabell DreissigChristoph Krafft
Mar 10, 2007·Journal of Cellular Physiology·Benjamin DrogatMichel Moenner
Aug 29, 2006·Trends in Biotechnology·Cyril Petibois, Gérard Déléris
Jul 25, 2015·IEEE Transactions on Bio-medical Engineering·Ugo BottoniAntonino S Fiorillo
Mar 10, 2012·Biotechnology Advances·Augusto MarcelliCyril Petibois
Sep 23, 2014·Acta Biomaterialia·Joseph BrownDavid L Kaplan
Jun 30, 2016·Applied Spectroscopy·Jopi J W MikkonenSami Myllymaa
Jun 5, 2018·Journal of Medical Engineering & Technology·Laís Morandini RodriguesJanete Dias Almeida
Nov 17, 2009·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M XingX Chen
Mar 27, 2019·International Journal of Legal Medicine·Kai ZhangZhenyuan Wang
Mar 30, 2017·Interdisciplinary Sciences, Computational Life Sciences·Su-Qin ZhouEn-Ren Wang
Nov 12, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Marcus A KramerAlbena Ivanisevic

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