Analytical, physiologic, and clinical validation of a radioimmunoassay for measurement of procollagen type III amino terminal propeptide in serum and bronchoalveolar lavage fluid obtained from dogs

American Journal of Veterinary Research
Simone SchullerKathleen McEntee

Abstract

To validate a radioimmunoassay for measurement of procollagen type III amino terminal propeptide (PIIINP) concentrations in canine serum and bronchoalveolar lavage fluid (BALF) and investigate the effects of physiologic and pathologic conditions on PIIINP concentrations. Sera from healthy adult (n = 70) and growing dogs (20) and dogs with chronic renal failure (CRF; 10), cardiomyopathy (CMP; 12), or degenerative valve disease (DVD; 26); and sera and BALF from dogs with chronic bronchopneumopathy (CBP; 15) and healthy control dogs (10 growing and 9 adult dogs). A radioimmunoassay was validated, and a reference range for serum PIIINP (S-PIIINP) concentration was established. Effects of growth, age, sex, weight, CRF, and heart failure on S-PIIINP concentration were analyzed. In CBP-affected dogs, S-PIIINP and BALF-PIIINP concentrations were evaluated. The radioimmunoassay had good sensitivity, linearity, precision, and reproducibility and reasonable accuracy for measurement of S-PIIINP and BALF-PIIINP concentrations. The S-PIIINP concentration reference range in adult dogs was 8.86 to 11.48 mug/L. Serum PIIINP concentration correlated with weight and age. Growing dogs had significantly higher S-PIIINP concentrations than adults, b...Continue Reading

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Citations

Jun 4, 2008·Biological Trace Element Research·Kazuyuki SuzukiRyuji Asano
Oct 26, 2013·BMC Veterinary Research·Runa RørtveitJohan Høgset Jansen
Oct 9, 2014·Journal of Veterinary Cardiology : the Official Journal of the European Society of Veterinary Cardiology·Melanie J HezzellJonathan Elliott
Jan 21, 2016·Journal of Veterinary Cardiology : the Official Journal of the European Society of Veterinary Cardiology·S SakarinS D Surachetpong
Apr 28, 2009·Journal of Veterinary Cardiology : the Official Journal of the European Society of Veterinary Cardiology·Adrian Boswood
Aug 19, 2007·The Veterinary Clinics of North America. Small Animal Practice·Cécile Clercx, Dominique Peeters

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