Analytical SEM of cell polyanions stained with tris(2,2'-bipyridine)ruthenium(II).

Micron : the International Research and Review Journal for Microscopy
J C Stockert, G E Bertolesi

Abstract

Peritoneal mast cells and lymphocytes from mice were placed on graphite supports, fixed in methanol, stained with the new fluorochrome tris(2,2'-bipyridine)ruthenium(II) and microanalysed using scanning electron microscopy (SEM). X-ray microanalysis showed the expected signal of P and S (K alpha lines) in emission spectra of lymphocytes and mast cells. The signal of Ru (L alpha 1 and L beta 1) overlapped with that of Cl, although the peak in the corresponding region was about 7 times higher than that from unstained cells. X-ray images showed the topographic localization of P, S and Ru in mast cells and lymphocytes and confirmed the accumulation of the Ru complex in heparin- and DNA-containing structures. These results indicate that, by using suitable marker elements and detection methods, analytical SEM is a useful complement in cytochemical studies.

References

Nov 1, 1976·Journal of Microscopy·G H HaggisR G Fulcher
Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Wu, N Davidson
Mar 1, 1986·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·T Ushiki, T Fujita
May 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G E BertolesiJ C Stockert

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Citations

Aug 30, 2008·Physics in Medicine and Biology·Akimasa HirataOsamu Fujiwara
Sep 14, 2012·Physics in Medicine and Biology·Ilkka Laakso, Akimasa Hirata
May 23, 2014·Physiological Measurement·F J PettersenJ O Høgetveit
Jul 28, 2017·Physics in Medicine and Biology·Elyar Ghalichi, Nevzat Güneri Gençer

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