Jul 31, 2008

Analytical speciation of chromium in in-vitro cultures of chromate-resistant filamentous fungi

Analytical and Bioanalytical Chemistry
Francisco Javier Acevedo AguilarKatarzyna Wrobel

Abstract

In this work, different analytical speciation schemes have been used to study the reduction of Cr(VI) by a chromate-resistant strain of filamentous fungi Ed8 (Aspergillus sp), indigenous to contaminated industrial wastes. As demonstrated previously, this strain has the capability to reduce chromate present in the growth medium without its accumulation in the biomass, yet the reduced chromium end-products have not been characterized. Liquid growth medium, initially containing 50 mg L(-1) Cr(VI), was analyzed for Cr(III)/Cr(VI) and for total Cr at different time intervals (0-24 h) after inoculation with fungi. Three hyphenated procedures, based on the Cr(III)-EDTA formation and species separation by anion-exchange or ion-pairing reversed-phase chromatography with ICP-MS or DAD detection were used. The results obtained for Cr(VI) in each case were consistent, demonstrating efficient reduction of chromate during 24 h of Ed8 growth. However, pre-column complexation with EDTA did not ensure complete recovery of the reduced forms of chromium in the above procedures. An alternative speciation scheme, based on extraction of Cr(VI)-benzyltributylammonium bromide (BTAB) ion pairs into chloroform and subsequent determination of residual ch...Continue Reading

Mentioned in this Paper

Chromium product (pharmacologic preparation)
Chromium
Filamentous fungus
Benzyltributylammonium bromide
Oxidation
Edetic Acid, Sodium Salt
High Pressure Liquid Chromatography Procedure
Inductively-Coupled Plasma Mass Spectrometry
Fungi
Aspergillus

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