PMID: 7371635Feb 1, 1980Paper

Analytical subcellular fractionation of rat liver with special reference to the localisation of putative plasma membrane marker enzymes

European Journal of Biochemistry
G D Smith, T J Peters

Abstract

A method for the subcellular fractionation of rat liver using whole homogenates of rat liver and analytical sucrose density gradient centrifugation is presented. The distributions in the sucrose gradients of marker enzymes for all organelles have been determined for control homogenates and for homogenates prepared in the presence of selective membrane perturbants. This technique is not subject to potential loss of information inherent in the use of postnuclear supernatants as starting material for fractionation experiments. Particular attention has been paid to the distributions of putative plasma membrane marker enzymes, up to 50% of which may be found in the nuclear pellet. Gamma-Glutamyltransferase has been found to be entirely plasma membrane in location but has a different distribution pattern when compared with other plasma membrane markers. Particulate alkaline phosphatase and alkaline phosphodiesterase are shown to have bimodal distribution, one peak of which is coincident with 5'-nucleotidase. The other peak is coincident with that of the golgi marker, galactosyltransferase, but the membrane structure containing these activities shows characteristics of plasma membrane rather than golgi apparatus.

References

Jan 1, 1976·Annual Review of Biochemistry·A Meister, S S Tate
Nov 1, 1977·Analytical Biochemistry·J Kapuściński, B Skoczylas
Mar 20, 1978·Biochimica Et Biophysica Acta·R HahnL Flohé
Sep 1, 1975·Canadian Journal of Biochemistry·I M YousefM M Fisher
Jul 15, 1967·International Journal of Cancer. Journal International Du Cancer·S Fiala, A E Fiala
Jul 1, 1971·The Journal of Cell Biology·C de Duve

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Citations

Mar 27, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·T ShahT J Peters
Apr 1, 1983·The Journal of Experimental Medicine·C Darte, H Beaufay
Apr 1, 1987·European Journal of Biochemistry·R J PeaseT J Peters
Aug 1, 1988·European Journal of Biochemistry·G B O'Beirne, D C Williams
May 1, 1989·European Journal of Biochemistry·G D SmithT J Peters
Feb 22, 1990·European Journal of Biochemistry·G B O'BeirneD C Williams
Apr 1, 1984·Journal of Clinical Pathology·F C MartinT J Peters
Aug 29, 2014·Molecular Biology of the Cell·Dan DouRobert Daniels
Feb 1, 1983·European Journal of Clinical Investigation·F C MartinT J Peters
Sep 1, 1985·Archives of Biochemistry and Biophysics·C W SlifeS Conroy
Nov 30, 1990·Biochemical and Biophysical Research Communications·J R ChristensenG D Smith
Oct 20, 1980·FEBS Letters·G D SmithT J Peters
Jul 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·I Ghadiminejad, H Baum
Nov 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A J Lança, Y Israel
Mar 1, 1985·Scandinavian Journal of Gastroenterology·C O'MorainT J Peters
Mar 1, 1993·The American Journal of Physiology·J FawcettG D Smith
May 1, 1990·Journal of Virology·T A JonesE Barklis
Feb 13, 1981·Biochimica Et Biophysica Acta·J L DingT J Peters
Oct 13, 1981·Biochimica Et Biophysica Acta·J L DingT J Peters
Jul 11, 1984·Biochimica Et Biophysica Acta·R J PeaseT J Peters
Jun 18, 1985·Biochimica Et Biophysica Acta·B K ChowdharyT J Peters
Jan 11, 1984·Biochimica Et Biophysica Acta·M BassetT J Peters

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