Analyzing Ash Leaf-Colonizing Fungal Communities for Their Biological Control of Hymenoscyphus fraxineus

Frontiers in Microbiology
Regina BeckerAndreas Ulrich

Abstract

The invasive ascomycete Hymenoscyphus fraxineus has been threatening Fraxinus excelsior populations throughout Europe for over two decades. Since the infection and first colonization by the pathogen occurs in leaves, leaf-colonizing microorganisms have been discussed as a barrier and as possible biocontrol agents against the disease. To identify fungal groups with health-supporting potential, we compared the fungal microbiota of compound leaves from susceptible and tolerant ash trees in four ash stands with high H. fraxineus exposure. The fungal communities were analyzed both culture-independently by ITS2 amplicon sequencing and by the taxonomic classification of 1,704 isolates using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) or sequencing of the entire ITS region. The fungal community structure did not show significant differences depending on the health status. However, for several OTUs and a MALDI group, a significantly higher abundance was found in tolerant ash trees. Thus, the yeast Papiliotrema flavescens was significantly increased and accounted for 12.3% of the mycobiome of tolerant ashes (OTU0003), and it had also a distinctly higher abundance among the isolates. The fil...Continue Reading

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Datasets Mentioned

BETA
PRJNA611938

Methods Mentioned

BETA
PCR
amplicon sequencing

Key Resources (RRID) Mentioned

SCR_011947
SCR_004891
SCR_015022

Software Mentioned

ANOSIM
vegan
Flex Control
R
MicrobiomeAnalyst
metagenomeSeq
BLAST
VSEARCH
MiSeq
UPARSE

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