Analyzing cell cycle checkpoints after ionizing radiation

Methods in Molecular Biology
Bo Xu, Michael B Kastan

Abstract

Several methods to measure cell cycle perturbation after exposure to ionizing radiation are presented in this chapter. These methods include the G1 checkpoint assay by 5' bromode-oxyuridine (BrdUrd) labeling followed by flow cytometric analysis, the S-phase checkpoint assay by measuring DNA synthesis in response to ionizing radiation, and the G2/M checkpoint assay by quantitating histone H3 phosphorylation after ionizing radiation.

Citations

May 30, 2009·Molecular Biology of the Cell·Ali PedramEllis R Levin
Jan 24, 2007·Molecular and Cellular Biology·Hiroyuki NiidaMakoto Nakanishi
Mar 1, 2012·Radiation Oncology·Ira K GordonKevin Camphausen
Apr 13, 2012·Breast Cancer Research : BCR·Ying YanKenneth H Cowan
Oct 2, 2007·Radiation Research·George D Wilson, Brian Marples

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