Analyzing metabolic variations in different bacterial strains, historical perspectives and current trends--example E. coli

Current Opinion in Biotechnology
Joseph ShiloachAlejandro Negrete

Abstract

The analysis of metabolic differences in bacterial strains is a useful tool for the development of strains with desired growth and production properties. Several methods are available for the evaluation and understanding of the differences: Biochemical methods to measure metabolites concentration and enzyme activity, mathematical methods to analyze metabolic fluxes through the various pathways, proteomic methods to identify expressed proteins, and genomic methods to detect and measure gene expression. A combination of the various methods is required to obtain a comprehensive understanding of metabolic activities. The genomic methods provide substantial amount information on global gene expression but do not always reflect the actual activity of the individual components. The review focuses on the different methodologies and their use, as well as historical overview of the evaluation of the differences between Escherichia coli K and E. coli B.

References

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Citations

Mar 28, 2012·Amino Acids·Zhao-Lai DaiWei-Yun Zhu
Sep 21, 2016·Applied Microbiology and Biotechnology·Vicente BernalManuel Cánovas
Aug 25, 2017·Journal of the Royal Society, Interface·Márcia R CoutoLígia R Rodrigues
Jun 7, 2021·BMC Bioinformatics·Yunfeng WangDaniel Gautheret

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