Analyzing shear stress-induced alignment of actin filaments in endothelial cells with a microfluidic assay.

Biomicrofluidics
A D van der MeerI Vermes

Abstract

The physiology of vascular endothelial cells is strongly affected by fluid shear stress on their surface. In this study, a microfluidic assay was employed to analyze the alignment of actin filaments in endothelial cells in response to shear stress. When cells were cultured in microfluidic channels and subjected to shear stress, the alignment of filaments in the channel direction was significantly higher than in static cultures. By adding inhibitory drugs, the roles of several signaling proteins in the process of alignment were determined. Thus, it is shown how microfluidic technology can be employed to provide a mechanistic insight into cell physiology.

References

Jul 1, 1987·IEEE Transactions on Bio-medical Engineering·S ChaudhuriC B Frank
Oct 11, 2002·The Journal of Pathology·Paul P M van ZuijlenEsther Middelkoop
Apr 30, 2003·The Journal of Cell Biology·Beata Wojciak-Stothard, Anne J Ridley
Jan 7, 2004·Journal of the Society for Gynecologic Investigation·D W BurleighT G Golos
Jul 1, 2005·Analytical Chemistry·Jonathan W SongShuichi Takayama
Aug 9, 2005·Journal of Biomechanics·Yi-Shuan J LiShu Chien
Sep 29, 2005·Journal of Cell Science·Chee Ping NgMelody A Swartz
Nov 26, 2008·Nature Clinical Practice. Cardiovascular Medicine·Peter F Davies
Apr 8, 2009·Lab on a Chip·Eugene TkachenkoAlex Groisman
Jun 18, 2009·Lab on a Chip·Lien ChauJustin Cooper-White

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Citations

Mar 6, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Andries D van der Meer, Albert van den Berg
May 8, 2013·Lab on a Chip·William J PolacheckRoger D Kamm
May 4, 2016·Tissue Barriers·Marinke W van der HelmLoes I Segerink
Oct 4, 2012·Trends in Neurosciences·Larry J Millet, Martha U Gillette
Sep 8, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroshi KamijoShuh Narumiya
May 30, 2015·Journal of Biomaterials Science. Polymer Edition·Xiangdong BiQian Wang
Mar 1, 2015·Microvascular Research·Adam ReinitzPeter C Searson
Sep 5, 2013·Advanced Drug Delivery Reviews·Kae SatoKiichi Sato
Mar 29, 2014·The Analyst·Roland ThuenauerWinfried Römer
Nov 20, 2016·Biotechnology and Bioengineering·Andrea P FalangaPaolo A Netti
Feb 23, 2017·Analytical Chemistry·Chunhong ZhengYanyi Huang
Sep 21, 2013·Vascular and Endovascular Surgery·Christof KarmonikHendrik von Tengg-Kobligk
Feb 16, 2018·Clinical Science·Isobel ParkesM Zameel Cader
Feb 27, 2019·Current Pharmaceutical Design·Beatrice MiccoliYi-Chen Ethan Li
Aug 21, 2019·Micromachines·Qian FengBenoît Kornmann
Jan 21, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cornelia BratengeierAnna Fahlgren
Sep 24, 2014·Biofabrication·Eric S HaldPatrick W Alford
Mar 15, 2018·Analytical and Bioanalytical Chemistry·Chengpeng ChenR Scott Martin
Oct 2, 2020·Micromachines·Sean BeverungRobert Steward
Jun 22, 2020·Seminars in Cell & Developmental Biology·Brady M TrevisanGraça Almeida-Porada
Feb 15, 2021·Journal of Neuroscience Research·Paul M HollowayMichele Zagnoni
Jan 29, 2021·Journal of Biomechanics·Sara Ben-SaadonDavid Elad
Apr 13, 2021·Journal of Physics D: Applied Physics·Christian M GriffithWilliam J Polacheck
Nov 27, 2021·Science Advances·John-Michael ArpinoJ Geoffrey Pickering

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Methods Mentioned

BETA
GTPase

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