Analyzing the Colocalization of MAP1LC3 and Lysosomal Markers in Primary Cells

Cold Spring Harbor Protocols
Kanchan Phadwal

Abstract

This technique evaluates the colocalization of the autophagy protein MAP1LC3 (microtubule-associated proteins 1A/1B light chain 3B, here referred to as LC3) with lysosomes (autolysosomes) in primary cells in a high-throughput manner. It uses an imaging fluorescence-activated cell sorting cytometer called the ImageStream to concomitantly detect surface molecules, making possible the identification of cells in mixed cell populations (e.g., in blood or bone marrow). It can be applied to clinical samples and to rare cell populations because only a few cells are needed for detection.

References

Sep 11, 2002·Molecular Biology of the Cell·Eeva-Liisa EskelinenPaul Saftig
May 12, 2010·Autophagy·Kai Er EngGerald M McInerney
May 23, 2012·American Journal of Human Genetics·Gabriela Lopez-HerreraBodo Grimbacher
Aug 5, 2015·Cold Spring Harbor Protocols·Suganthi ChittaranjanSharon M Gorski
Aug 5, 2015·Cold Spring Harbor Protocols·Nicholas T Ktistakis
Sep 4, 2015·Cold Spring Harbor Protocols·Daniel PulestonAnna Katharina Simon

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Citations

Aug 2, 2017·Redox Biology·Ioanna KorovilaTobias Jung

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