Analyzing the platelet proteome

Seminars in Thrombosis and Hemostasis
Angel GarcíaSteve P Watson

Abstract

During the last 10 years, mass spectrometry (MS) has become a key tool for protein analysis and has underpinned the emerging field of proteomics. Using high-throughput tandem MS/MS following protein separation, it is potentially possible to analyze hundreds to thousands of proteins in a sample at a time. This technology can be used to analyze the protein content (i.e., the proteome) of any cell or tissue and complements the powerful field of genomics. The technology is particularly suitable for platelets because of the absence of a nucleus. Cellular proteins can be separated by either gel-based methods such as two-dimensional gel electrophoresis or one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by liquid chromatography (LC) -MS/MS or by multidimensional LC-MS/MS. Prefractionation techniques, such as subcellular fractionations or immunoprecipitations, can be used to improve the analysis. Each method has particular advantages and disadvantages. Proteomics can be used to compare the proteome of basal and diseased platelets, helping to reveal information on the molecular basis of the disease.

Citations

Oct 5, 2007·Blood·David CerviGiannoula L Klement
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Aug 29, 2007·Journal of Thrombosis and Haemostasis : JTH·D M MaynardW A Gahl
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Apr 21, 2012·International Journal of Peptides·Dolores Javier Sánchez-GonzálezNayeli Isabel Trejo-Bahena
Feb 21, 2006·Blood Cells, Molecules & Diseases·Angel García
Apr 1, 2010·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Imran AliBhavtosh Sharma
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