Analyzing the Transcriptome Profile of Human Cumulus Cells Related to Embryo Quality via RNA Sequencing

BioMed Research International
Qiwei LiuZhigang Xue

Abstract

Selecting excellent oocytes is required to improve the outcomes of in vitro fertilization (IVF). Cumulus cells (CCs) are an integral part of the oocyte maturation process. Therefore, we sought to identify differentially expressed genes in CCs to assess oocyte quality and embryo development potential. We divided the participants' embryos into the high-quality embryo group and low-quality embryo group by the information including age, body mass index, and the levels of luteinizing hormone, follicle-stimulating hormone, estradiol, and progesterone. We analyzed a total of 7 CC samples after the quality control in RNA sequencing. We found that 2499 genes were unregulated and 5739 genes were downregulated in high-quality embryo group compared to the low-quality embryo group (Padj < 0.05). Interestingly, MSTN, CTGF, NDUFA1, VCAN, SCD5, and STAR were significantly associated with the quality of embryo. In accordance with the results of RNA sequencing, the association of the expression levels of MSTN, CTGF, NDUFA1, VCAN, SCD5, and STAR with the embryo quality was verified by quantitative reverse-transcription polymerase chain reaction (RT-qPCR) in 50 CC samples. Despite the small sample size and lack of validation in animal models, our ...Continue Reading

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Citations

Jan 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nikola SekulovskiJames A MacLean
Mar 20, 2021·Reproductive Biomedicine Online·Caroline M Da LuzPaula A Navarro
Oct 14, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·R Ariel Igal, Débora I Sinner
Aug 19, 2021·Zygote : the Biology of Gametes and Early Embryos·Elisabeth Schmidt da SilvaPaulo Bayard Gonçalves
Oct 14, 2021·Reproductive Sciences·Azam GovahiMehdi Mehdizadeh

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Methods Mentioned

BETA
RNA-Seq

Software Mentioned

SPSS

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