Anandamide activates vanilloid receptor 1 (VR1) at acidic pH in dorsal root ganglia neurons and cells ectopically expressing VR1

The Journal of Biological Chemistry
Zoltan OlahMichael Iadarola

Abstract

The vanilloid receptor type 1 (VR1) is a heat-activated ionophore preferentially expressed in nociceptive neurons of trigeminal and dorsal root ganglia (DRG). VR1, which binds and is activated by capsaicin and other vanilloid compounds, was noted to interact with the endocannabinoid anandamide (ANA) and certain inflammatory metabolites of arachidonic acid in a pH-dependent manner. At pH < or = 6.5 ANA induced (45)Ca(2+) uptake either in primary cultures of DRG neurons or cells ectopically expressing C-terminally tagged recombinant forms of VR1 with an EC(50) = approximately 10 microm at pH 5.5. Capsazepine, a potent antagonist of vanilloids, inhibited ANA-induced Ca(2+) transport in both cell systems. Vanilloids displaced [(3)H]ANA in VR1-expressing cells, suggesting competition for binding to VR1. Ratiometric determination of intracellular free calcium and confocal imaging of the VR1-green fluorescent fusion protein revealed that, at low pH (< or =6.5), ANA could induce an elevation of intracellular free Ca(2+) and consequent intracellular membrane changes in DRG neurons or transfected cells expressing VR1. These actions of ANA were similar to the effects determined previously for vanilloids. The ligand-induced changes in Ca(2...Continue Reading

References

Feb 1, 1991·Annals of the Rheumatic Diseases·C R StevensD R Blake
Mar 1, 1991·Trends in Neurosciences·B Lynn
Dec 1, 1994·Trends in Neurosciences·S Bevan, P Geppetti
Jun 14, 1996·Neuroscience Letters·M KressL Vyklicky
Aug 1, 1996·Spine·J T RobertsonR P White
Mar 13, 1997·Nature·R WaldmannM Lazdunski
Aug 22, 1997·The Journal of Biological Chemistry·R WaldmannM Lazdunski
Apr 16, 1998·Pain·Jennelle Durnett RichardsonKenneth M Hargreaves
May 26, 1998·Brain Research. Brain Research Reviews·P B Simpson, J T Russell
Aug 11, 1998·Neuron·M J Berridge
Nov 4, 1998·FEBS Letters·V Di MarzoL De Petrocellis
Jan 27, 1999·European Journal of Pharmacology·M Beltramo, D Piomelli
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·A ZimmerT I Bonner
Nov 5, 1999·Trends in Pharmacological Sciences·O Sterner, A Szallasi
Feb 9, 2000·Trends in Pharmacological Sciences·J Szolcsányi
Feb 9, 2000·Trends in Pharmacological Sciences·P M ZygmuntE D Högestätt
Feb 29, 2000·British Journal of Pharmacology·D SmartJ B Davis
Mar 31, 2000·Trends in Pharmacological Sciences·D Smart, J C Jerman
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·S W HwangU Oh
Jun 6, 2000·Trends in Pharmacological Sciences·D PiomelliF Rodríguez de Fonseca
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·S E JordtD Julius
Jun 24, 2000·British Journal of Pharmacology·J C JermanD Smart

❮ Previous
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Citations

Dec 21, 2002·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Lu-Yuan LeeChin-Yin Ho
May 28, 2005·Archives of Pharmacal Research·Soon-Youl LeeUhtaek Oh
Jun 1, 2005·Hearing Research·Sigrid E Myrdal, Peter S Steyger
Sep 11, 2002·European Journal of Pharmacology·Akiyoshi SomeyaToshihiko Murayama
Jul 16, 2003·European Journal of Pharmacology·Vera Ralevic
Apr 5, 2002·Trends in Pharmacological Sciences·Martin J GunthorpeJohn B Davis
Aug 17, 2002·British Journal of Pharmacology·Manabu KagayaDomenico Spina
Nov 2, 2002·British Journal of Pharmacology·Vadim N DedovBasil D Roufogalis
Sep 26, 2009·The Journal of Biological Chemistry·Sonia Gallego-SandínJavier García-Sancho
Feb 11, 2010·The Journal of Biological Chemistry·Shu WangHuai-hu Chuang
Apr 24, 2007·Cephalalgia : an International Journal of Headache·M J M Fischer, K Messlinger
Oct 19, 2011·The Journal of Pharmacology and Experimental Therapeutics·Krisztian KaszasMichael J Iadarola
Oct 15, 2008·PloS One·László PeczeZoltán Oláh
Dec 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Tej K PareekAshok B Kulkarni
Jun 19, 2004·Molecular Membrane Biology·Abraham B ChangMilton H Saier
Jul 2, 2005·Expert Opinion on Emerging Drugs·J B CalixtoA R Santos
Sep 25, 2012·Molecular Pain·Evan E LebovitzMichael J Iadarola
Nov 21, 2009·Journal of the American Academy of Dermatology·Christine B WarrenEdward V Maytin
Sep 2, 2008·Pharmacology, Biochemistry, and Behavior·Gabor TubolyGyöngyi Horvath
Mar 14, 2007·Pharmacology & Therapeutics·Katarzyna StarowiczVincenzo Di Marzo
Dec 17, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·E S KimballP J Hornby
Oct 1, 2003·British Journal of Pharmacology·Ruth A Ross
Mar 17, 2004·British Journal of Pharmacology·Rhian M EvansRuth A Ross
May 17, 2008·CNS Neuroscience & Therapeutics·Richard P Shank, Bruce E Maryanoff
May 4, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·L ZhiW Rong
May 22, 2009·Cranio : the Journal of Craniomandibular Practice·Wesley E Shankland
Mar 17, 2009·Brain Research·Carl PotenzieriDonald A Simone
Aug 5, 2011·Channels·Cadie L Buckley, Alexander J Stokes

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